FastQCFastQC Report
Tue 11 Oct 2022
A729_Gastroc_S8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA729_Gastroc_S8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171385
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATGA409.094947E-1295.036321
AGTTTGA800.095.036321
GTTTCGA409.094947E-1295.036321
GGCACGA201.7405377E-595.036321
GTTAGCA409.094947E-1295.036321
GCGTTGA201.7405377E-595.036321
TGTGCGA201.7405377E-595.036321
AGGTTCA409.094947E-1295.036321
TTTGGTA800.095.036321
TTTGGGA800.095.036321
AGTTCGA201.7405377E-595.036321
TAAGGTA800.095.036321
GTGCGTA409.094947E-1295.036321
TCGGTTA409.094947E-1295.036321
AGGTGGA409.094947E-1295.036321
TGCACGA201.7405377E-595.036321
GTCGGCA201.7405377E-595.036321
TGATCGA650.095.0363161
TTAGGTA950.095.0363161
TAGGTTA950.095.0363161
Produced by FastQC (version 0.11.4)