FastQCFastQC Report
Tue 11 Oct 2022
A729_Diaphragm_S14_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA729_Diaphragm_S14_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125189
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20640.18343584944396008No Hit
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16590.14744189642806677No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATG254.653357E-794.88741
TTAGGGG850.094.88741
AGCCGGG500.094.88741
TGGAGAG500.094.88741
GATAAGG254.653357E-794.88741
GATCGGG254.653357E-794.88741
ATAGGAG254.653357E-794.88741
AAGAGGA550.094.88742
GAAGACT1850.094.88744
GAATCCA44950.094.88747
ACCCTTG254.653357E-794.88741
AGGCGAA1000.094.88743
ACGGGAC254.653357E-794.88743
GTAATGA500.094.88742
AATGACC500.094.88748
TGCGAGG254.653357E-794.88741
CGAGGAC254.653357E-794.88743
TGCCTGG254.653357E-794.88741
GCTAGAC254.653357E-794.88743
GCCTAGT550.094.88742
Produced by FastQC (version 0.11.4)