FastQCFastQC Report
Tue 11 Oct 2022
A728_SpCord_S41_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA728_SpCord_S41_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852650
Sequences flagged as poor quality0
Sequence length101
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGC254.6495916E-794.896972
AGCCGGC201.7529896E-594.896973
GGTACAC201.7529896E-594.896974
GGAGCAC500.094.896974
TAGACTG254.6495916E-794.896971
GGCACAC8150.094.896976
GATCCTG201.7529896E-594.896971
GCTGCAC353.3287506E-1094.896974
GCCATGC201.7529896E-594.896972
ATACTGC353.3287506E-1094.896972
TGCATGC409.094947E-1294.896972
GTACTGC409.094947E-1294.896972
AGACAAC409.094947E-1294.896977
GGCGGAC550.094.896974
GCAATGC500.094.896972
GACGCAC409.094947E-1294.896974
CGCACAT1100.094.896976
GTCTTGC353.3287506E-1094.896972
TTGCAGC353.3287506E-1094.896972
GAATTGC409.094947E-1294.896972
Produced by FastQC (version 0.11.4)