FastQCFastQC Report
Tue 11 Oct 2022
A728_SpCord_S41_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA728_SpCord_S41_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852650
Sequences flagged as poor quality0
Sequence length101
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG8770.10285580249809417No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGGA600.095.027121
AGCGTTA301.2290911E-895.027121
AATCCGA156.609832E-495.027121
ACCGGTA156.609832E-495.027121
CCGGGAA156.609832E-495.027121
TTTACGA301.2290911E-895.027121
GGATGAA301.2290911E-895.027121
TTTGTCA156.609832E-495.027121
TAGGTTA301.2290911E-895.027121
TAGCGGA156.609832E-495.027121
TCGGGGA301.2290911E-895.027121
GCGTTTA156.609832E-495.027121
CGTTCTA156.609832E-495.027121
CGTTCAA156.609832E-495.027121
CTGCGTA156.609832E-495.027121
GTTACGA156.609832E-495.027121
AATGCGA156.609832E-495.027121
CGTAGGA156.609832E-495.027121
CGAGGGA156.609832E-495.027121
GCGGGTA156.609832E-495.027121
Produced by FastQC (version 0.11.4)