FastQCFastQC Report
Tue 11 Oct 2022
A728_Liver_S35_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA728_Liver_S35_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6818958
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCATC2800.094.909587
GTCATCC156.6444435E-494.909572
GGACTCT409.094947E-1294.909576
GCGCCAT1350.094.909576
ATTCCGC1050.094.909572
GCATTGC2600.094.909572
GGTGACT156.6444435E-494.909574
GCAATCT156.6444435E-494.909576
GGATTCC201.7527183E-594.909572
CGCTTCC301.2410965E-894.909572
AGTCCCG750.094.9088751
GGATCCG254.649628E-794.9088751
ATCCGCG301.2410965E-894.9088751
AGCATTG2850.094.9088751
CAGGCCG254.649628E-794.9088751
ACGTACG353.3287506E-1094.9088751
CGATACG254.649628E-794.9088751
AGGTCCG254.649628E-794.9088751
AGCGATG1600.094.908871
GGACATT304400.094.8472067
Produced by FastQC (version 0.11.4)