FastQCFastQC Report
Tue 11 Oct 2022
A728_Liver_S35_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA728_Liver_S35_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6818958
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG87670.12856803048207657No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGGT353.3105607E-1094.991457
GTGATGC156.6217093E-494.991447
GCGTAGC201.7452305E-594.991447
GTGCTAC1350.094.9809952
GATGCAT500.094.9809952
TTTGCAC1100.094.9809952
AGGGCAT550.094.9809952
GTTGCAC1100.094.9809952
TAGCGAT353.3105607E-1094.980992
AGGGTAC1700.094.980992
GTAGCAC850.094.980992
TGCGATT1600.094.942693
GGGAACT2500.094.942693
TGCAGTT2050.094.942694
GATTTGC4950.094.942686
AGTGGCT1754500.094.93737
AGTGCCT113200.094.907537
AGTTGCT76600.094.867447
GTGGCTA1735450.094.8573468
AAGTGGC568650.094.850866
Produced by FastQC (version 0.11.4)