FastQCFastQC Report
Tue 11 Oct 2022
A728_FrCx_S47_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA728_FrCx_S47_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659813
Sequences flagged as poor quality0
Sequence length101
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGCC254.6476453E-794.899593
GCCTTGC254.6476453E-794.899592
GGAGCCC500.094.899594
ATCTAGC254.6476453E-794.899592
GCGAATG254.6476453E-794.899591
CGGCCAA1000.094.899596
TGGCCAT13900.094.899596
GTGGACA14600.094.899595
AACATGC500.094.899592
AACTGCC500.094.899593
GATGCAC1700.094.899594
TGAGCCC850.094.899594
GCATCTG254.6476453E-794.899591
TGACTGC254.6476453E-794.899592
GACTAGC254.6476453E-794.899592
GTCATAC156.644539E-494.899582
ATGGGCT1950.094.899585
AGACTGC156.644539E-494.899582
GCCCATA3150.094.899587
TTGGCAC4300.094.899584
Produced by FastQC (version 0.11.4)