FastQCFastQC Report
Tue 11 Oct 2022
A728_FrCx_S47_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA728_FrCx_S47_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659813
Sequences flagged as poor quality0
Sequence length101
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG7400.1121529888013725No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA201.7392245E-595.044531
TCGTTCA201.7392245E-595.044531
TCTTGCA201.7392245E-595.044531
ATAGGGA201.7392245E-595.044531
TTTGTTA850.095.044531
CGGTTTA201.7392245E-595.044531
CGTTGTA201.7392245E-595.044531
ATTCGTA550.095.044531
TTCGTGA409.094947E-1295.044531
GGTTTGA409.094947E-1295.044531
TTCCGGA201.7392245E-595.044531
AGTGGGA201.7392245E-595.044531
GTAGGGA201.7392245E-595.044531
TGACCGA201.7392245E-595.044531
GAGCGAA201.7392245E-595.044531
GTTTTTA550.095.044531
TAAGGTA201.7392245E-595.044531
AGGGGTA201.7392245E-595.044531
TTGTTTA550.095.044531
GCGCTTA201.7392245E-595.044531
Produced by FastQC (version 0.11.4)