FastQCFastQC Report
Tue 11 Oct 2022
A728_DRG_S53_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA728_DRG_S53_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820832
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGC600.094.897142
GCCTTGC1050.094.897142
GGGAGCA850.094.897143
ATCGTGC254.6523382E-794.897142
ATCCGCC254.6523382E-794.897143
GAGCCCG156.647496E-494.897141
ACGTAGC500.094.897142
GCTCCGC156.647496E-494.897142
CGCCATT254.6523382E-794.897147
GGCCATA13850.094.554547
GGGACAT46950.094.391836
GGCACAA53200.094.362016
ACACCGC94450.094.3445369
TGGCCAT36600.094.248936
GACACCG38800.094.163398
GGCTGTA32050.094.156918
CCACCGC91450.094.1188669
GGGGACA58400.094.084665
GGCCATT36850.093.9958047
TGGACAT54200.093.934166
Produced by FastQC (version 0.11.4)