FastQCFastQC Report
Tue 11 Oct 2022
A728_DRG_S53_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA728_DRG_S53_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820832
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTTA1300.095.024561
GTGCGTA650.095.024561
GTTTGCA1050.095.024551
AGCGTTA750.095.024551
GGATGTA1100.095.024551
TGTGGCA750.095.024551
GGAACGA353.2923708E-1095.024551
TAGGGCA600.095.024551
GCAGGCA254.6153218E-795.024551
TGCGTTA800.095.024551
GAAGGTA700.095.024551
AGGGTTA900.095.024541
TAGGTAC850.094.98582
GGTTTAC850.094.98582
AGGGTAC650.094.98582
TTTGGAC850.094.98582
TAGGGAC700.094.98582
GGGTTAC850.094.98582
AGCGTAC409.094947E-1294.9857942
AACCGAC201.7455674E-594.9857942
Produced by FastQC (version 0.11.4)