FastQCFastQC Report
Tue 11 Oct 2022
A727_TA_S21_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA727_TA_S21_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983570
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCGC201.7527855E-594.9006652
AGGAGCA353.3287506E-1094.9006653
AATGGAC8400.094.9006654
TGAGTGC353.3287506E-1094.9006652
GCCATGC353.3287506E-1094.9006652
ACGATGG409.094947E-1294.9006652
GTTCGAC201.7527855E-594.9006654
GATGCAC3000.094.9006654
GCCTAGC201.7527855E-594.9006652
TTCATGC850.094.9006652
AGGATGC201.7527855E-594.9006652
CATCCAC353.3287506E-1094.9006654
AGTAAGC353.3287506E-1094.9006652
GAGGCAC3000.094.9006654
GAAATGC550.094.900662
AAGTAGC550.094.900662
AGACTGC550.094.900662
AGTCCGC254.649046E-794.900662
GAAGCAC2550.094.900664
GTCGATG156.645217E-494.900661
Produced by FastQC (version 0.11.4)