FastQCFastQC Report
Tue 11 Oct 2022
A727_TA_S21_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA727_TA_S21_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983570
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG13380.1360350559695802No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTA800.095.028141
GTCATGA201.7411421E-595.028141
TTTAGGA500.095.028141
CGTGGTA201.7411421E-595.028141
TCTTGGA409.094947E-1295.028141
GGTACGA201.7411421E-595.028141
GATGTGA600.095.028141
CTCCGAA156.6098536E-495.028141
GGATGGA301.229273E-895.028141
GGATGCA201.7411421E-595.028141
CGTGCGA201.7411421E-595.028141
GCGCGAA156.6098536E-495.028141
TCTTCGA353.2923708E-1095.028141
CGTTGGA254.6120294E-795.028141
TTATGGA700.095.028141
GGTCCGA201.7411421E-595.028141
GGTCCAA156.6098536E-495.028141
GTTAGGA409.094947E-1295.028141
GTAGGTA550.095.028141
AGGCGTA156.6098536E-495.028141
Produced by FastQC (version 0.11.4)