FastQCFastQC Report
Tue 11 Oct 2022
A726_TA_S2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA726_TA_S2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504157
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8180.16225104481342123No Hit
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7010.1390439882814282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCCA5850.094.89317
GACTCAA6000.094.89317
GGCAGAC254.6479363E-794.893093
CAACGAA254.6479363E-794.893093
ATGGAGA450.094.893092
GGACTCC3600.094.893096
GCTATCC254.6479363E-794.893096
AGAAGCG254.6479363E-794.893091
AGAAGAA500.094.893093
CTCCACC11150.094.893099
CTAAGAC254.6479363E-794.893093
TTTCCGA254.6479363E-794.893092
AGACGTC254.6479363E-794.893093
TGCGAAT1750.094.893094
ACCAGTC254.6479363E-794.893093
ACGTGAG254.6479363E-794.893091
GTACTTG254.6479363E-794.893091
TTGCGTC254.6479363E-794.893093
GAAAATC500.094.893095
ACTGTTG254.6479363E-794.893091
Produced by FastQC (version 0.11.4)