FastQCFastQC Report
Tue 11 Oct 2022
A726_TA_S2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA726_TA_S2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504157
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGACAATCGCTGCGTCAATGGGGGGGGGGGGGGGGGGGGGGGGGG7760.15392030657116731No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCA201.7381433E-595.0520251
GTGTTGA409.094947E-1295.0520251
AACGTTA201.7381433E-595.0520251
TGTTTCA254.601843E-795.0520251
TTTAGCA201.7381433E-595.0520251
GGGTGTA254.601843E-795.0520251
TTAGGGA201.7381433E-595.0520251
TCGTTGA201.7381433E-595.0520251
TCGTTCA254.601843E-795.0520251
GCCTTAA201.7381433E-595.0520251
GAGCTTA201.7381433E-595.0520251
ATGATGA353.2923708E-1095.0520251
GGGCTTA254.601843E-795.0520251
TTCAGGA201.7381433E-595.0520251
GGTCGGA254.601843E-795.0520251
GGATGTA254.601843E-795.0520251
GCTGGTA201.7381433E-595.0520251
TGAAGGA201.7381433E-595.0520251
GGGTCTA201.7381433E-595.0520251
TTTGTGA409.094947E-1295.0520251
Produced by FastQC (version 0.11.4)