FastQCFastQC Report
Tue 11 Oct 2022
A725_TA_S1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA725_TA_S1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492187
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9410.19118749580951955No Hit
GGGGGGGCTGGACGGGGCGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6790.1379556957010242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAA650.094.8926243
TACTGAA650.094.8926243
AGGTCAG254.647918E-794.892621
ACGCGAG156.645466E-494.892621
ACGCGAA201.7526492E-594.892623
ATTTACG201.7526492E-594.892621
TATCACC156.645466E-494.892628
GTGCACA254.647918E-794.892625
AAGTACG254.647918E-794.892621
GGCAGTC201.7526492E-594.892623
TAGAGGA156.645466E-494.892622
AACAACC700.094.892629
AATCCTC11850.094.892628
GAGTGAG156.645466E-494.892621
TAGAGAG301.240187E-894.892621
TGGAGAC156.645466E-494.892623
GATACGG254.647918E-794.892621
TTGGCGA156.645466E-494.892622
GGCAGAC254.647918E-794.892623
GGCAGAA450.094.892623
Produced by FastQC (version 0.11.4)