FastQCFastQC Report
Tue 11 Oct 2022
A725_TA_S1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA725_TA_S1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492187
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAA156.6031434E-495.045261
CTTGTGA254.6036257E-795.045261
TCTGCAA156.6031434E-495.045261
CTTCGTA156.6031434E-495.045261
TTGATGA156.6031434E-495.045261
AACGTTA254.6036257E-795.045261
GTGTTCA156.6031434E-495.045261
TTTAGGA201.738717E-595.045261
TTAGGTA301.2263627E-895.045261
AACGTGA201.738717E-595.045261
ATGGGGA201.738717E-595.045261
TTAGGGA353.2923708E-1095.045261
TCGTTTA301.2263627E-895.045261
CAGCGAA156.6031434E-495.045261
AGTCCTA156.6031434E-495.045261
GGCAGGA156.6031434E-495.045261
TCGTTCA156.6031434E-495.045261
TCGTTAA254.6036257E-795.045261
CCGGGTA156.6031434E-495.045261
GTACCGA156.6031434E-495.045261
Produced by FastQC (version 0.11.4)