FastQCFastQC Report
Wed 31 Jul 2019
i712_i502_S10_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamei712_i502_S10_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55707064
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGG1310850.2353112703983107No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA1066780.1914981554224434No Hit
CGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCTGGGCCT1053480.1891106664677212No Hit
CGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGC753930.13533831185215578No Hit
GCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCC734250.1318055462409579No Hit
CCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTC715490.12843793024166558No Hit
GCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAG675600.12127725848197636No Hit
GCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGA623850.11198759281228679No Hit
GGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGT615400.11047072952902347No Hit
CGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGC606560.108883857171148No Hit
CCGCTACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACT586190.10522722935102091No Hit
CGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCC570760.10245738314264775No Hit
CCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCG564980.1014198127548061No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)