FastQCFastQC Report
Sun 3 Mar 2019
69_S9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename69_S9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20239441
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT6276553.1011479022567867TruSeq Adapter, Index 7 (97% over 36bp)
CGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCT792680.39165113305253835No Hit
GGGACGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCA497510.24581212494949833No Hit
GTCCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGT400110.19768826619272736No Hit
CGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCACCGC365690.1806818676464434No Hit
GGGAGGACGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGCGGGG341640.16879912839489986No Hit
CGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGCGGGGAGGGAGC337640.16682278922624394No Hit
GGGAGGACGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGTGGGG297590.14703469330007682No Hit
CGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGTGGGGAGGGAGC286150.14138236327772097No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA278500.13760261461766657No Hit
GGACGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGCGGGGAGGG268090.13245919193123962No Hit
GGACGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGTGGGGAGGG232100.1146770802612582No Hit
CTCCGAGGCCCCGCCCGTCCTCCTCGCCCTCCCCGCGCGTACGCGCGCGC230960.11411382359819128No Hit
CCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCC226310.1118163293146288No Hit
GGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGCGGGCGGGC222860.11011173678166308No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATGTCGTAT218140.10777965656264914TruSeq Adapter, Index 7 (97% over 36bp)
CGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGG215040.10624799370694082No Hit
CGCGGGGCGGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGC209430.10347617802290093No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG208890.1032093722351324No Hit
CGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAGAGAGAGAG206970.10226072943417756No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203400.10049684672615218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)