FastQCFastQC Report
Sun 3 Mar 2019
65_S5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename65_S5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48324322
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1196710.24764134300735768TruSeq Adapter, Index 13 (97% over 38bp)
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA945700.1956985552740916No Hit
CGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAGAGAGAGAG753360.15589665179368684No Hit
CGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCT532420.1101764035096033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)