FastQCFastQC Report
Sun 3 Mar 2019
64_S4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename64_S4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90265647
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT9041801.0016878292580123TruSeq Adapter, Index 7 (97% over 38bp)
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA1616820.179117976077876No Hit
CGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCT1610140.17837793817619232No Hit
GGGAGAGCGAGCGGGGCCGTGCCCGGCGGCGCGGAGCGGCGCGGCGGAGG1344810.14898358840766965No Hit
GTCCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGT1154830.12793682185649208No Hit
CGCGGGGCGGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGC1107310.12267236061577225No Hit
CGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAGAGAGAGAG1064310.11790864358397607No Hit
CCCGAACCCACCGCGATCGCTCACACGGCCCGCGCGCACCCGCCAGAGGG949110.10514631330344312No Hit
CCGGGGGTTCCCGCCCCCACGGCGCGGGGCGCACGCCACACGCGCGGCAG925280.1025063277948919No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)