FastQCFastQC Report
Sun 3 Mar 2019
63_S3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63_S3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31670169
Sequences flagged as poor quality0
Sequence length76
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT9973323.149121180881605TruSeq Adapter, Index 2 (97% over 37bp)
CGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCT1533420.48418434394840143No Hit
GTCCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGT992770.3134716458254454No Hit
GGGACGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCA991910.3132000969113869No Hit
CGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCACCGC991540.31308326772743145No Hit
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA628450.19843594772102416No Hit
GTCGGGAGGGGCCCGGCCCCTCCCGCGCCTCCACCGCGGACTCCGCTCCC625740.19758025288718856No Hit
CCGGGGGTTCCCGCCCCCACGGCGCGGGGCGCACGCCACACGCGCGGCAG616680.1947195166530371No Hit
CGCGGGGCGGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGC545830.17234830669833182No Hit
CCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCC493840.1559322275798402No Hit
CCCGAACCCACCGCGATCGCTCACACGGCCCGCGCGCACCCGCCAGAGGG466990.14745421787929203No Hit
GGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGCGGGCGGGC466010.1471447784190858No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA459310.14502922292583914TruSeq Adapter, Index 2 (97% over 37bp)
CGGGGGTTCCCGCCCCCACGGCGCGGGGCGCACGCCACACGCGCGGCAGG433510.13688275550408335No Hit
CGACGCGCCGCGAGGCGAGCCGGGCGGGCGGGCGCGCGCGCGTACGCGCG428900.13542712702290918No Hit
GGGAGAGCGAGCGGGGCCGTGCCCGGCGGCGCGGAGCGGCGCGGCGGAGG391650.12366526998956022No Hit
CGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCCTCGGAGG370530.11699653386756477No Hit
CGGCGAGCGAGAGGCGGACCGCGGTGCCCGGCCCGGGGACAGTCGCGCCG369900.11679760850028935No Hit
CGCCCGTCCGCCCGTGGGTCGGGGGCGGTGGTGGGCCCGCGGGGGAGTCC366870.11584087221005988No Hit
CGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCC356730.11263912106057913No Hit
CCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCTTCTC340850.10762493878703332No Hit
GCGGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGCGGGCGG330220.10426846790744944No Hit
CGCGCGGCCACCTCGAGCGTTCGCGTTCAGGGCGGGGGCCCGGCCGGTGC325020.10262654424104903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)