FastQCFastQC Report
Sun 3 Mar 2019
62_S2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename62_S2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37787921
Sequences flagged as poor quality0
Sequence length76
%GC77

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT2795740.7398501759332037TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT2144620.5675411462832263TruSeq Adapter, Index 6 (97% over 36bp)
GGGAGGACGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGTGGGG1540750.4077361122883686No Hit
CGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGTGGGGAGGGAGC1427860.3778614864787084No Hit
CGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCT1104210.29221242417649806No Hit
GGACGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGTGGGGAGGG1081440.28618668912745954No Hit
GTCCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGT888200.23504865483337917No Hit
GGGAGAGAGAGAGAGAGAGGGCGCGGGGTGGGGAGGGAGCGAGCGGCGCG851750.22540271532800124No Hit
CGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAGAGAGAGAG820130.21703496204514666No Hit
GGGACGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCA709090.18764991066854406No Hit
CGCGGGGCGGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGC668350.1768686877481299No Hit
GGGGAGGACGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGTGGG645940.17093822123741606No Hit
GGAGGACGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGTGGGGA645300.170768854947061No Hit
CGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCACCGC605370.16020198623787743No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG595850.15768266266884595TruSeq Adapter, Index 6 (97% over 36bp)
CTCCGAGGCCCCGCCCGTCCTCCTCGCCCTCCCCGCGCGTACGCGCGCGC594750.1573915643572982No Hit
GGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGCGGGCGGGC559960.14818491866752873No Hit
GGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGTGGGGAGGGAGCG543170.1437416998939952No Hit
GCGGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGCGGGCGG514750.13622077806291594No Hit
CGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAGAGAGAG493070.13048349497713832No Hit
CGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCCTCGGAGG461990.12225864450177082No Hit
CGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCC454350.12023683441065731No Hit
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA436280.11545488305641373No Hit
CGACGCGCCGCGAGGCGAGCCGGGCGGGCGGGCGCGCGCGCGTACGCGCG426210.11279001033160833No Hit
CCCGCCGCCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGG404320.106997153931808No Hit
AGGACGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGTGGGGAGG389390.1030461559396189No Hit
GGGAGGACGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGCGGGG388890.10291383852527901No Hit
CCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCC387840.10263597195516526No Hit
CTCCTCCGAGGCCCCGCCCGTCCTCCTCGCCCTCCCCGCGCGTACGCGCG386740.1023448736436175No Hit
CGCGGGGTGGGGAGGGAGCGAGCGGCGCGCGCGGGGTGGGGCGGGGGAGG386080.10217021465668884No Hit
CGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGCGGGGAGGGAGC381960.10107991916252816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)