FastQCFastQC Report
Thu 11 Jul 2019
59_S5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename59_S5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75526911
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG1694160.2243120998288941No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT1470870.19474780320354954No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA1418690.1878390074764212No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC1312280.1737499895898033No Hit
GAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACT1300240.17215585581144713No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA1254720.16612886498164872No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT1226860.16244011356428967No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA1210080.1602183889130591No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG1190060.15756767809555988No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG1176770.15580804039503218No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA1168760.15474749126175702No Hit
TGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGC1167600.15459390362198183No Hit
CGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCG1155280.15296269696505926No Hit
GCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAA1153330.15270451084647166No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT1126570.14916140288062357No Hit
CGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGG1110680.14705751702197908No Hit
CCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCT1109290.14687347666052433No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGG1103330.1460843539596105No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTT1102820.14601682835936453No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT1080760.14309601514088136No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT1044900.13834803862162454No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG1029440.1363010861122071No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC1021700.13527628582612097No Hit
GTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAG950030.12578695294449418No Hit
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGG923800.12231401864164682No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT920410.12186517200471762No Hit
TAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTT900010.11916414799487828No Hit
GGAACCGCAGGTTCAGACATTTGGTGTATGTGCTTGGCTGAGGAGCCAAT880690.11660611937379511No Hit
CCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACC873230.11561839196627545No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT865360.11457637927228348No Hit
GGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATT844930.11187138316831202No Hit
CCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCAC799320.10583247605611727No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT795980.10539024957607496No Hit
CACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAG787700.10429395159561074No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA774210.1025078332675356No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT765310.1013294453416743No Hit
GGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGC758380.10041189159715534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)