FastQCFastQC Report
Sun 3 Mar 2019
52_S1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename52_S1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19425452
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG435980.22443750601015616No Hit
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA387640.1995526281705054No Hit
CGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAGAGAGAGAG370020.19048205416275513No Hit
CGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCC243100.12514509314892647No Hit
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA235090.12102163697400709No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA231840.11934857423137439No Hit
CGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCT211500.10887777540517461No Hit
CGACGCGCCGCGAGGCGAGCCGGGCGGGCGGGCGCGCGCGCGTACGCGCG209890.10804896586190117No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT208750.10746210693063925TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)