FastQCFastQC Report
Sun 3 Mar 2019
51_S3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename51_S3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52521862
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2740850.5218493586537355TruSeq Adapter, Index 2 (97% over 37bp)
CGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCT2250660.4285186995084066No Hit
GGGACGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCA1539250.29306843691109047No Hit
GTCCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGT1106510.21067607999122345No Hit
CGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCACCGC1073640.20441773370487132No Hit
CGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGCGGGGAGGGAGC871760.16598040640676448No Hit
CCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCTTCTC749820.14276340774057095No Hit
GGGAGGACGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGCGGGG721960.13745894995116512No Hit
CCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCC712400.13563875553383845No Hit
CGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGTGGGGAGGGAGC686150.13064083676241334No Hit
CGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAGAGAGAGAG667620.12711278210205115No Hit
GGACGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGCGGGGAGGG637880.12145037813015844No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG629240.11980534886596365No Hit
CGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGG619190.11789185996490376No Hit
GGGAGGACGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGTGGGG579190.11027598374177974No Hit
GCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCTTCT575480.109569611222085No Hit
CTCCGAGGCCCCGCCCGTCCTCCTCGCCCTCCCCGCGCGTACGCGCGCGC556500.10595587795421267No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA549130.10455265276010207TruSeq Adapter, Index 2 (97% over 37bp)
CGCGGGGCGGGGAGGGAGCGAGCGGCGCGCGCGGGGTGGGGCGGGGGAGG531330.10116358784081189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)