FastQCFastQC Report
Sun 3 Mar 2019
50_S2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename50_S2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123
Sequences flagged as poor quality0
Sequence length76
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21.6260162601626018No Hit
CGGTGGTCGCCGTATCATTAAAAATGAAGAGCGTCGTGTTGGGAAAGAGT10.8130081300813009Illumina Single End PCR Primer 1 (96% over 25bp)
CAAGAATAAACCCACAACAGATATTCACCTTGTTTCGCCAGACGCATTGC10.8130081300813009No Hit
GCGGGAGTTTTGCGTGGTGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAT10.8130081300813009Illumina Single End PCR Primer 1 (96% over 27bp)
CAAGCGGAAGGGGGGGGGCGGGGGTGGCGGGGGTGGGGGGGGGGGGGGGG10.8130081300813009No Hit
CCCGCACTACTCGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC10.8130081300813009Illumina Single End PCR Primer 1 (96% over 33bp)
CAAACAAAAGATGGAAAAACAACTTCAGAGTCGTTGACGGCAAGCTAATC10.8130081300813009No Hit
CGAGATCGGAAGAGCGTCGTGTATGGAAAGAATTTATTAGCCTTTGTAGA10.8130081300813009Illumina Single End PCR Primer 1 (96% over 29bp)
CGATCACATTGGGACTGAGAAACGTCCCAGACTACTACGGGAGGCAGCAG10.8130081300813009No Hit
CGTATGCCTTCTTCATATTGAGATCTGAAGAGCGTCGTGTAAGGAAAAAG10.8130081300813009Illumina Single End PCR Primer 1 (95% over 21bp)
CAAGAAACAGTAGGCAAATCGTTAATACATTCGTGCAAGATCGGAAGAGC10.8130081300813009No Hit
CGAAAGTCTGACGGAGCAACGCCGAGTGAGTGATGAAGATCGGAAGAGCG10.8130081300813009No Hit
CCTAAGCCGAGGCGGAAAAGCGTAGGCGATGGACAACAGGTTGAAATTCC10.8130081300813009No Hit
CACCAAAAAGAACGCAAAAGCGTCTATAATGCAAAAAATGTATTAACCAA10.8130081300813009No Hit
CCTACAAAAGTAGGTACAACAAGTTCACAGTCGTTGACGGCAATCTAATC10.8130081300813009No Hit
TCGAAAGCGACTCTGAACAGGGCGTTCAGTTAGTAATAGTAGACCCGAAA10.8130081300813009No Hit
CTGCCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGGCTTCATTT10.8130081300813009No Hit
CACAGATTGTGAATGTGAATATAAACTGAACTACACCCTGCGCAACACAG10.8130081300813009No Hit
CTAAGTCTGTCGCATTTCATGTTGAAAACAGAAGGTAGTGCTCCTAGAAA10.8130081300813009No Hit
AAGAAGTTGGCTTGCAAGCAGCCATTAATTCAAAGAGTGCGTAACAGATC10.8130081300813009No Hit
CCACGCCCGGGGCACCCCCCTCGCGGCCCGCCCCCCCCCCCCCCCCCCCC10.8130081300813009No Hit
CGAACTCAGACGTACTCCTTAAAACATGCAAGTCGAACGATGAAGCCTAG10.8130081300813009No Hit
GCCTTTCGTGCCGGTCGGAACTTACCCGACAAGGAATTTCGCTAGATCGG10.8130081300813009No Hit
AATTCTGCAACTCAATAATGCTAAAATCGTTGCTGCACTTCACACTACAT10.8130081300813009No Hit
CACACACATTAAATCATGGCTGCTTCTAAGCCAACATCCTGGTTGTTTTG10.8130081300813009No Hit
CGTGACTAGATCGGAAGAGCGTCGTGTAGGGAAAGACTGTCTTAGCCTGT10.8130081300813009Illumina Single End PCR Primer 1 (96% over 33bp)
CCTCGAGACGCCCTAGCGGGAAGGCCGGGGAGAGCGAGCGGGGCCGTGCC10.8130081300813009No Hit
AACTTCTTCACCCTCACGACTACATCTCAAGACACTCGTCTAAGAAAAAA10.8130081300813009No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATTCGCCTGTGTATATA10.8130081300813009Illumina Single End PCR Primer 1 (97% over 34bp)
CCAAAGGCATGCTCAGGCCGACAGACGGCAGATCGGAAGAGCGTCGTGTA10.8130081300813009Illumina Single End PCR Primer 1 (100% over 21bp)
CTATAAACAACAAACACAGGAGCATGAAAAACCTAAAGCTGATGTATAGG10.8130081300813009No Hit
ATCCAAGGGAACAGGGAGGGAGCGAGAGATCCTGCGAGCGAGAAGCGGGG10.8130081300813009No Hit
CAACAACCATGCACCAACTGTATAAGATCGGAATAGCGTCGTGTATGGAA10.8130081300813009Illumina Single End PCR Primer 1 (95% over 21bp)
TAGAAAGCGAGTCTGAACAGGGCGTTCAGTTAGTAATAGTAGACCCGAAA10.8130081300813009No Hit
ATTATGTTACAGAAATTACAAGGTAACATTTCCTTCGATAATCATCTGTC10.8130081300813009No Hit
GATGATCCTTACTGGAGCGAGAATAAAAAGTTGTCTCTAGATACAGATGC10.8130081300813009No Hit
CCAACAAAAGTTGGTACAACGACTTGAGAGTCGGTGACGGCAAGCTAATC10.8130081300813009No Hit
GGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTC10.8130081300813009No Hit
AAAGTTTCCCATCTTTGTGCAGCTACCTCCGCATTGCTGTGTAGTGACCC10.8130081300813009No Hit
CAAGCAAACGTATTCAACGCAGCTTTGCAGATCTGCGATTACTAGCGACT10.8130081300813009No Hit
CTCCGCCATAAGTCTAAGGTTTCCCGGGCAATGCCAATCAGCCCGGGGTA10.8130081300813009No Hit
CGCAATACTACAATATCATCTGACAAGAGCATAGCTGGGTAGCTATGTAG10.8130081300813009No Hit
CAACCAAAATAATGCATAAGAGCATATCATGAAAAACCGTTTTCCCCGCG10.8130081300813009No Hit
GTCAAACACCATCTCTGATGCGGTACAGATCGGAAGAGCGTCGTGTAAGG10.8130081300813009Illumina Single End PCR Primer 1 (95% over 24bp)
TTGAACCCCCATTTCCTTAATTATTTCAGGAATAATTCTCCCGAAATCAG10.8130081300813009No Hit
CAGAAATTGACAGATCGGAAGAGCGTCGAGTAGTGAAAGAGTGTCTTCGC10.8130081300813009Illumina Single End PCR Primer 1 (95% over 22bp)
CAAGCAGAATAATCCTAACATCAATCTAAATCGTCATCTTCACAGATACT10.8130081300813009No Hit
CCTACAAAGGTTGGAACAACGAGTTGCGAGTCGTTGACGGCAAGCTAATC10.8130081300813009No Hit
CAATCCTCACAAACAATACACGAAACCACTCAAAACTCCAACGCCCACGA10.8130081300813009No Hit
CGGCCGACCGCCGCTCCCGCGCCCCTCCTCCTCCCCCCCCGCCCCACCTC10.8130081300813009No Hit
AGCATATCCCAGAGTTAAAGGTAGTTAACTCACGTATTACTCACCCGTTC10.8130081300813009No Hit
AGGTTCTTCGCCTTGCATCGAATTAATCCGCATGCTCCGCCGCTTGTGCG10.8130081300813009No Hit
TGGCGAAGATCGGAAGAGCGTCGTGTAGGGAAATAGTGTCTTCGCCTGTG10.8130081300813009Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGCCCCGCCCCGACTCCCCCCGCCGCCGCGGCCCCCCCGCCCGCCCCC10.8130081300813009No Hit
CAAAAATAATACTCGATAAAGTTAAGACCCGAAAGAAAGGTGTAACGACT10.8130081300813009No Hit
CGGCGCGGCGCGGCCCCGGCCGGGGAGCGGAGTCCGCGGTGGAGGCGCGG10.8130081300813009No Hit
CGGGGCGGGGAGGGAGCGAGCGGCGCGCGCGGGGTGGGGCGGGGGAGGGC10.8130081300813009No Hit
AAACATTCAGACGAATTAATTCTAACAAATCATTTCGCTACTCATACCGA10.8130081300813009No Hit
AAACAATAAGACGAAATAATTCTAACAATTCATTTCCCTACTCATACACA10.8130081300813009No Hit
ATCCGATGTGTAGGATAGTCCGGAGGCTTTGAATAGATCGGAAGAGCGTC10.8130081300813009No Hit
CGCTTATAAAGTCAATACTCAGATACCCAGCGGTGCACCTGGCGGCACAA10.8130081300813009No Hit
GTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGGTGAGATCTGAAGAG10.8130081300813009No Hit
GGGGCGGGAGACGGCGGAGGGGGTTATAAGGATTTTGTTGGATTATAAAA10.8130081300813009No Hit
AAAGAAAATGGCTCAAATACCGGATAAGACCAATCTCCGCATGGGGTGTT10.8130081300813009No Hit
CCGGGGGTAACCTCTGGTGGATTGTGTGGGAGTGAGAATGCAGGCATGAG10.8130081300813009No Hit
CAAAAAGAATACGGCAAACAATAAACTCCTAACTAATCCCGTTAGCTCCC10.8130081300813009No Hit
GTTTGATTCTGGCTCAGGACGAGATCGGAAGAGCGTCGTGTATGGAAAAA10.8130081300813009Illumina Single End PCR Primer 1 (96% over 27bp)
CAAAAAAAAAACCCCAAACAAGATTCAAAAAAAACGAAAAAAAAAATGAA10.8130081300813009No Hit
TGGTTCTTCGCGTTGCATCGAATTAATCCGCATGCTCCGCCGCTTGTGCG10.8130081300813009No Hit
TGCTGCGTTGATCAGACAAAATTTCGTCCATTGCGGAAGATTCCCCACTG10.8130081300813009No Hit
NNNATGTAACAGAAATTACAAGGTAACATTTCCTTCGATAATCATCTGTC10.8130081300813009No Hit
GGGGAGGAGACGGTTCCGGGGGACCGGCCGCGACTGCGGCGGCGGTGGTG10.8130081300813009No Hit
CCCGCTCAAACAGCCATCCTAAAAAATACCGACAGGTCGTTACTCGGCCC10.8130081300813009No Hit
CCTTGATCGCCGTATCATTAAAAAAGAAGAGAGTCGAGTAGGGTAACACT10.8130081300813009No Hit
AAATCAGCCACTCAATAAAGCTCAAATCCTTCCTGCACTTCACACTACAT10.8130081300813009No Hit
CGGCCGCAAGGCTAAAACTCAAAGAAATTGACGGGGGCCCGCACAAGCGG10.8130081300813009No Hit
CTTTACGTCCCATCCATACTAATATCAATCTTCACCAGATCGGAAGAGCG10.8130081300813009No Hit
AAAGCAAAAACAGGCCTTAAAGATAAACCAGACTGTGACTTAAGTGAGGT10.8130081300813009No Hit
GGGGGGCGCGGGAGCGGAGGAGCGGCGCGGGAGTATATGACAGGGGGGGG10.8130081300813009No Hit
AAAAAAGAAATAATCAGATCGAATAGACACGAGTAGGGAAAGATTTTCTT10.8130081300813009No Hit
AGGGATTAGACCCGAAACCGGGTGACCTAACCATGAGATCGGAAGAGCGT10.8130081300813009No Hit
CAAAAAGTCCCCTAATAGAAATCTAAACAGTGGTACTAAGCGTACGAAAA10.8130081300813009No Hit
ACGCTGAGGAGCGAAATACAGGGGAGAGAACGGGATTAGATACCCCGGTA10.8130081300813009No Hit
CACGGATAAACGCTGAAAGCATCTAAGCGTGAAACCAGCCTTGAGAGATC10.8130081300813009No Hit
GGGAGGACGGCAATCGAGATTCTCCGGACTTGCATGTAATAGTAATTAAT10.8130081300813009No Hit
CTGACATCGCAACACCGTCGTGAATGTAAACAGTGTCATAGCCTGAGTAA10.8130081300813009No Hit
CCCGCCTAAGACTGTCAACCCGGTTGTAAATCGTCCTGTTAGAAATCAAT10.8130081300813009No Hit
AAACAATCAGACGAATTAATACAAACAAATCATTTCGCTACTCATACCGA10.8130081300813009No Hit
CACGCATAAACAGCCATAACAACATCAACCCCTGTCTTTAGTTGACAACA10.8130081300813009No Hit
CAATCATATAACCATATACAGGATTAACCAACGTTAAGACTTTGTCGAGG10.8130081300813009No Hit
CAACCACACAGCACCAATCGACCATATACCTATTACGCTTTCTTTAAATG10.8130081300813009No Hit
GAACTCTCCACCCCAAATCAACAGTGTACATTTTTTTCAGCACCACACCA10.8130081300813009No Hit
CTGGGAGAGGACGGCGTAGGAGAGTAAAAGGAGTGAATAGAATAAGTAAG10.8130081300813009No Hit
CAGTCACATTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAG10.8130081300813009No Hit
GGGAGGACGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGTGGGG10.8130081300813009No Hit
CAACCACAAAACACCAAACGACCATATACCTATTACGCTTTCTTTAAATG10.8130081300813009No Hit
CAATAAACAACAAACAAACGAGCATCAAAAAACTAAAGCTGATGTATAGG10.8130081300813009No Hit
GGAAACAATGCTGCTAGCGACATTCAAGCTGTGCGCTGGGAGCTCCTACA10.8130081300813009No Hit
ATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGGTGGTCTCAAAC10.8130081300813009No Hit
AAAGCAGATGACATACACCCTCGAACAAAGACTAAGGTTTCCCGGGCAGA10.8130081300813009No Hit
AAATCCCCCTTCCTATATTATAATGGATCATGCGATATCAGGATGGGGAA10.8130081300813009No Hit
GGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.8130081300813009No Hit
AGAAAACTGACGCTGAGGCGCGAAAGCCAGGTGAGCGAACGGGATTAGAT10.8130081300813009No Hit
AATATATTATCCATCAGGACTAGATAGCAAGACACTCGTATAAGGAAAAA10.8130081300813009No Hit
GAAGTAAGCCTATGCATCAATCCGGATTTGCGAGTCACAGTGGCAGTTCT10.8130081300813009No Hit
CAATCCTCACAAACAATTCACGAAACAACTCAAAACTCCAACGCCCACGA10.8130081300813009No Hit
CACTCATATCCTTGCAAACCCCATAATATCGCAAGACAGAAATTAAATGA10.8130081300813009No Hit
CAATACTTTACTTACATCAGTCCCATTGGCCGTACATATACGTTCTCCTT10.8130081300813009No Hit
AACCAAACGTGCCCTCTGCCCGATTGGAAACCGGCACATCGGAAGAGCGT10.8130081300813009No Hit
TGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAA10.8130081300813009No Hit
ATGGATGTCGTCAGCTAGTGCAGATCGGAAGAGAGTCGTGTATGGAAAGA10.8130081300813009Illumina Single End PCR Primer 1 (95% over 21bp)
CCTACATCGGAAGAGCGTCGTGTATGTAAAGAGTGTCTTAGCCTGTGTAG10.8130081300813009Illumina Single End PCR Primer 1 (95% over 21bp)
AAAGCAAAAACAGGCCGTAAAGATAAACCAGCCTGTGACTTAAGTGAGGT10.8130081300813009No Hit
GTGGCGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGG10.8130081300813009No Hit
AAAGCAGAAGACATTAAACCTCGAACAAAGACTAAGGTTTCCCGGGCAGA10.8130081300813009No Hit
CAAGCCTAAAAATCCATAACATATTTAGCCAGAGTGATCATCTGAGGTCA10.8130081300813009No Hit
CTTCCTCACAGGACGACTCGACCAGTGACCTATTACGCTTTCTTTAAATG10.8130081300813009No Hit
CAAAAAGATTACGGCAAGCACTACAGTGCTATCTAATCCCGTTAGCTCCC10.8130081300813009No Hit
CAGGCAGATTTGCGTGGTGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAG10.8130081300813009Illumina Single End PCR Primer 1 (100% over 28bp)
CAAGCATTAAGTGCCATAACGAAATCAACCCCTTTCTTTAGTTTCCATCA10.8130081300813009No Hit
CACTTAAAAAGTCCAGAATCAGATACCCAGCGGTGCACCTGGCGGCACAA10.8130081300813009No Hit
CTACCAGAAGAGAAGAATCCAGAAAATCCGCTAAGGTCCCGAAATAATAG10.8130081300813009No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)