FastQCFastQC Report
Sun 3 Mar 2019
50_S2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename50_S2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGACGCTCTTCCGATCTGAACGTATTCACCGCGGCGTGCTGATCCGCG43.2520325203252036No Hit
CGTCCTGAGCCAGAATCAAACAGATCGGAAGAGCACACGTCTGAACTCCA32.4390243902439024Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAGGAGTTGTGGACTGATGAGAAGTGAGAATGTCGGTATGAGTAGCGAA32.4390243902439024No Hit
AAGTGGTTCCTCGGGAGACAGACAGCGGGTGCTAACGTCCGTTGTCAAGA32.4390243902439024No Hit
GGTCTCTATCTATCGTGGGCGTTGGAGTTTTGAGTGGTGCCGTCACTAGT21.6260162601626018No Hit
TGGACGAAAACTGACGCTGAGGCGCGAAAGCCAGGGGAGCGAACGGGATT21.6260162601626018No Hit
CGGCACCACGCAAAACTCCCGCAGATCGGAAGAGCACACGTCTGAACTCC21.6260162601626018Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT21.6260162601626018Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCCACCTTCCTCACACCTTACGGTGGCAGTATCTCCAGAGTGCCCAGC21.6260162601626018No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTATCTCAGTCCCAATGT21.6260162601626018No Hit
TTCCGATCTCTATCGTGGGCGTTGGAGTTTTGAGTGGTGCCGTCACTAGT21.6260162601626018No Hit
GAATACGTGGAAACGAACATAGTATTCTGACTGGAAACAGTAGACAGATG21.6260162601626018No Hit
CAACCTGGCCATGGATAGATCACTACGGTTTCGGGTCTACTATTACTAAC21.6260162601626018No Hit
ATAGGTAAATCCGTAGCTCTAAAAGCTGAGATGTGATGACGAGTTACTAG21.6260162601626018No Hit
CCGATCTACGGCCGCAAGGCTAAAACTCAAAGAAATTGACGGGGGCCCGC21.6260162601626018No Hit
GCCCGGGAAACCTTAGTCTTGCGGCGAGGGGGAATCTCACCCCCTTTAGA21.6260162601626018No Hit
AAAGTACTCTAGCGACCCAGTATGAAATGCAATTCCCAGGTTAAGCCCGG21.6260162601626018No Hit
TTCAGAGCTTCGACCGAAGTCTAACCCCTCCTCTTAACCTTCCAGCACCG21.6260162601626018No Hit
ATTCAAAGCCTCCGGCCTATCCTACACATCGGATAGATCGGAAGAGCACA10.8130081300813009No Hit
TCGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAT10.8130081300813009TruSeq Adapter, Index 6 (97% over 36bp)
CGCCGCGCCGGCTCCCCGTCTTCGGGGCCGGCCGGATTCCCGTCGCCTCC10.8130081300813009No Hit
GAGGCGTGGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCC10.8130081300813009No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTTGGCG10.8130081300813009TruSeq Adapter, Index 6 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGTTT10.8130081300813009TruSeq Adapter, Index 6 (97% over 36bp)
TATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTC10.8130081300813009No Hit
CACGACGAGCTCGACTTCAAACTCGTCTTTGGCGAGGACGGGGCGCCCGC10.8130081300813009No Hit
AGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGG10.8130081300813009TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGCACTCCAG10.8130081300813009Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGCATTGATTTTTTTTGATTTTTGATTTTTTTTGTTTTTTTTTTTTTATA10.8130081300813009No Hit
AGTCACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAA10.8130081300813009TruSeq Adapter, Index 6 (97% over 36bp)
CAGTTTCCCAGGCTTATCCCAGAGTTAAAGGTAGGTTACTCACGTATTAC10.8130081300813009No Hit
CATCCCAAGAGCACACGACTGAACTCCAGTCACACCGGAGAATCGGCGCG10.8130081300813009No Hit
TGCACGAATGGAGTAACGACTTCCCTACTGTCTCAACAGATCGGAAGAGC10.8130081300813009No Hit
GCCGCTTGTGCGGGCCCCCGTCAATTTCTTTGAGTTTTAGCCTTGCGGCC10.8130081300813009No Hit
TCCGCCGCCGAACCTGGTTAATCCACGTGCTAGGGGTGGGGCCCCCCAGG10.8130081300813009No Hit
CGTGGGTCGGGGGCGGTGGTGGGCCCGCGGGGGAGTCCCGTCGGGGGGGG10.8130081300813009No Hit
CTAAGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGA10.8130081300813009TruSeq Adapter, Index 6 (97% over 36bp)
GAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAG10.8130081300813009No Hit
CCCTACACGACGCTCTTCCGATCTTGTCGTTATGATGGAAAAGATAATTA10.8130081300813009Illumina Single End PCR Primer 1 (100% over 24bp)
GCCGGTTACCAATCGGCCCGAGGGCACCTTTGGAAAGATCGGAAGAGCAC10.8130081300813009No Hit
CAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCGGAGAATCCG10.8130081300813009TruSeq Adapter, Index 5 (97% over 37bp)
GCACACGTCTGAACTCCAGTCACTACCTGCAGGGGGGGGGTGCATCGGGG10.8130081300813009TruSeq Adapter, Index 10 (96% over 28bp)
GTTGCCACAGCTTCGGCGGTACGCTTGAGGGATCGGAAGAGCACACGTCT10.8130081300813009Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCCGAGTAGTGCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC10.8130081300813009Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGCAGCAAGCCAGAAAGCCACTCTGCAAGCAAACAGATACTCTGCA10.8130081300813009No Hit
GATCCGGACGCTGTCTCAACCATGATCTCAGTGAAATTGTAGTATCGGTG10.8130081300813009No Hit
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGG10.8130081300813009TruSeq Adapter, Index 6 (97% over 36bp)
CCGATCTCTCGCCTTAGGACCCGACTAACCCAGGGCGGATAAACCTGGCC10.8130081300813009No Hit
TTTCCCTACACGACGCTCTTCCGATCTGATCGGAAGAGCACACGTCTGAA10.8130081300813009Illumina Single End PCR Primer 1 (100% over 27bp)
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGTTGGTTTGGGGGG10.8130081300813009No Hit
CCATGAAGTCGGAGTCGCTAGTAATCGCAGATCAGCAACGCTGCGGTGAA10.8130081300813009No Hit
CAGGCATAGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCAAGGC10.8130081300813009No Hit
CGTCTCGCTCCCCTCGTACTTCTCGCCTGTCTGATTCTCTAAGTTCATTT10.8130081300813009No Hit
CCCCCCCCCCCCCCCCGGGGCCCCCCCCCCCGCGCGCGGGCGGCGCCCCC10.8130081300813009No Hit
GCACGAGCTGACGACAACCATAGATCGGAAGAGCACACGTCTGAACTCCA10.8130081300813009Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGTTTCAACCTTGCGGTCGTACTCCCCAGGCGGGGTACTTATTGCGTTA10.8130081300813009No Hit
GATTCTCACCATTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT10.8130081300813009Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT10.8130081300813009TruSeq Adapter, Index 3 (97% over 34bp)
AAACGGCTCACCAAGGCGACGACGGAGATCGGAAGAGCACACGTCTGAAC10.8130081300813009Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGGGCACCCCCCGCGCCGCCCTCCCCCCCCCCCCCCCCCCCCCCGC10.8130081300813009No Hit
GGTACTTCAATTTCACCGGGTCCCTCGTTGAGACAGTGCGCAAGTCGTTA10.8130081300813009No Hit
GCCGCCCCGACCCTTCTCCCCCCGCCGCCGCCCCCACGCGGCGCTCCCCC10.8130081300813009No Hit
TACTTTTCGTACGCTTAGTACCACTGTTGAGATTTCGAATACGGGACTAT10.8130081300813009No Hit
GCTTAACACATGCAAGTCGAACGATGAAGCCTAGCTTGCTAGGTGGATTA10.8130081300813009No Hit
CTGCCATCGCCCTCGCCCTTCGGCTGAGACTTAGGACCCGACTTACCCCG10.8130081300813009No Hit
CACGAAACAGTCAGTAAGGGACCCTCTGCACTGGGCTTCCTGGTGTCATT10.8130081300813009No Hit
ACTTAGCTATTATTTCGGGACCTTAGCCGATGGTCTGGATTCTTCTCCTC10.8130081300813009No Hit
GTCAATTTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGG10.8130081300813009TruSeq Adapter, Index 6 (97% over 36bp)
TATACAGGTGGTGCATGGTTGTCGAGATCGGAAGAGCACACGTCTGAACT10.8130081300813009Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACCTTCATGGGGTCGAGTTGCAGACCCCAATCCGAACAGATCGGAAGAG10.8130081300813009No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGG10.8130081300813009TruSeq Adapter, Index 6 (97% over 36bp)
CCAACACCCCATGCGGAGATTGGTCGTATCCGGTATTAGACCCAGTTTCC10.8130081300813009No Hit
CTCAAGGCTGGTTTCACGCTTAGATGCTTTCAGCGTTTATCCGTCAGATC10.8130081300813009No Hit
GGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10.8130081300813009No Hit
TACACGACGCTCTTCCGATCTGTTAGCTCAATGACTCACATCACTTACAC10.8130081300813009Illumina Single End PCR Primer 1 (100% over 21bp)
CGGAAGAGCACACGTCTGAACTACAGTCACTACCTGCTTGCTGGGAGGGG10.8130081300813009TruSeq Adapter, Index 10 (96% over 32bp)
CTTCATTTCATTATGTACCCAGTAATCATTCAGGAGCAGGTTGTTCTGTT10.8130081300813009No Hit
ACGCGCTCCCCCGACCCTCTCTCCCCGCCGGCACCCTTCCCCTTCCGGAC10.8130081300813009No Hit
TGCAGGTTACCCGCGACTGGACAGAAAGACCCCATGGAGCTTTACTGCAA10.8130081300813009No Hit
TGCCGTCATCGAAGACTATGCGGTATTAGTCTGCCTTTCGGCAGGTTATC10.8130081300813009No Hit
GGAAGGGGACGCCACGGGGACCCCTCGAGCGCGGCCGACCGCAGCCGGGA10.8130081300813009No Hit
GTTACGCACTCTTTGAATGAATGGCTGCTTCCAAGCCAACATCCTAGATC10.8130081300813009No Hit
GATCAGGCTGGTCTCGAACTCCTGACCTCAGATGATCCCCCTGCCTCCCA10.8130081300813009No Hit
CGAGGCGCCGCCGCCCCCCCCCCCCCCCCGGGGCCCCCCCCCCCCCGGCG10.8130081300813009No Hit
TAAGATTGAACTTAGTTAATGAATGTACAGGATAGGTGGGAGACGTAGAA10.8130081300813009No Hit
TATTTCTAGGAGCACTACCGTCTGTTTTCAACATGAAATGCCACACACAT10.8130081300813009No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTGGGGGGGGG10.8130081300813009TruSeq Adapter, Index 6 (96% over 32bp)
TGACCGCATCAGAGATGGTGTTTCCCAGATCGGAAGAGCACACGTCTGAA10.8130081300813009Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGTGGCGGC10.8130081300813009TruSeq Adapter, Index 6 (96% over 30bp)
GGTGAAGATGCCGATTACCCGCGATGGGACGAAAAGAGATCGGAAGAGCA10.8130081300813009No Hit
CATGGTTAGGTCACCCGGTTTCGGGTCTAATCCCAAGATCGGAAGAGCAC10.8130081300813009No Hit
GTTACACTAAGTGGCGAAGGATGTGGAATTTCCAAAACAACCAGGATGTT10.8130081300813009No Hit
TCATCACTCACGCGGCGTTGCTCCGTCAGACTTTCGAGATCGGAAGAGCA10.8130081300813009No Hit
CGCCTTTCACCCCTACCCACAGCTCATCCCCCAGGTTTTCAACCCTGGTG10.8130081300813009No Hit
CCCCGTCTACCTGTGTCGGTTTTGGTACGGGCGATGTGTCTCTACCTAGA10.8130081300813009No Hit
CCTCTACGGCCGACCATTCCAAGTCGTTCACCTACCATCCTGAACATCAT10.8130081300813009No Hit
AGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCAGATCGG10.8130081300813009No Hit
AATGAAGCTTGAGCCAACCCCACTTTCGCGGGGAAAACGGTTTTTCAACC10.8130081300813009No Hit
GGTTAGACTTCGGTCGAAGCTCTGAATTGAAGCCCCAGTAAACGGCGGCC10.8130081300813009No Hit
GCCGTCTGTCGGCCTGAGCATGCCTTTGGAGATCGGAAGAGCACACGTCT10.8130081300813009Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)