FastQCFastQC Report
Sun 3 Mar 2019
45_S7_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename45_S7_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT730.434782608695656TruSeq Adapter, Index 7 (97% over 36bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT28.695652173913043TruSeq Adapter, Index 2 (97% over 35bp)
CCTGGTAGTGAGAATTTGGTACAATATCTTAGTTACTTATCTGCACCATA14.3478260869565215No Hit
AAAAAAAATCAACAAACTGAAGTGACAACCACAGAATGGGAGAAAATATT14.3478260869565215No Hit
GTCGTGTGTGGGTTGACTTCGGAGGCGCTCTGCCTCGGAAGGAAGGAGGT14.3478260869565215No Hit
AGGTAAGCAGTACTTTTACCTTATCATGCAGTGCTCTACTCTTCTTTGCA14.3478260869565215No Hit
TCTGTCTCTACTAAAAATTCACAAAATTAGCCGGGTGTCGTGGCGGGGGC14.3478260869565215No Hit
CCGGGGCAACACCACCCAGGGGAACGAGGTCACCTCCATCCTGCGCTGGG14.3478260869565215No Hit
ATGGCATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGTACGGA14.3478260869565215No Hit
ACGGTCGGCGGGCGGCGGGGCCGGGCGGGTAGTGTCCCCCCCCCCCCCCG14.3478260869565215No Hit
CCGCGATCGCTCACACGGCCCGCGCGCACCCGCCAGAGGGGAGCACGGGA14.3478260869565215No Hit
CCCAGCTCTTCGGGAGGCTGAGACAGAAGAGTCGCTTGAATACAGGAGGT14.3478260869565215No Hit
GCACCGACTGCCGCCAGTAGAGCGGGGCGCTCATGAGATCGGAAGAGCAC14.3478260869565215No Hit
AGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTC14.3478260869565215No Hit
CGAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAGATCGGAAGAGCACAC14.3478260869565215No Hit
CTCACGCCTGTAATCCCAACATTTTGGGAGGCTGAGGTGGGCGGATCACT14.3478260869565215No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)