FastQCFastQC Report
Sun 3 Mar 2019
41_S5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename41_S5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCGCGCGCCCAGCCCCGCTTCGCGAGATAGGAAGAGCGTCGTGTAGG15.263157894736842Illumina Single End PCR Primer 1 (95% over 23bp)
TGGCAGTAGCTGCCCGTGAAGCCGGGTGGACACAGGCAGGTGAACGAGTT15.263157894736842No Hit
CTCATGATCTCCTGACAAACTGATCTCACTTCAAAGCCCTCCCAAAGTGA15.263157894736842No Hit
GATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTA15.263157894736842No Hit
GCCTGTAATCCCAGCACTTGGGGAGGCTGTGGCAGGTGGAGCACTTGAGG15.263157894736842No Hit
TGGGACTGCACTCGCCCACCACCACACCTCGCTAATATTTCTATTTTTGG15.263157894736842No Hit
GCTGAATCAATTTGATAGGATTAATCTCCAGTGAAGCTGTGTTTACAGGG15.263157894736842No Hit
AAAAAATAAAACATGTGGAAACATACATAAACACAAAAAAGCCCACAAAA15.263157894736842No Hit
AGATGAATAATAATTTAAACTCACAGTACAGATAATCAAACCACCTACAT15.263157894736842No Hit
GGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTACCTCACCT15.263157894736842No Hit
CGCAGACCCGCCGGCGTCCGCCCTCCTCTTTCAGCCGCGCCCGCCCCATC15.263157894736842No Hit
ATTTTTCTTAATGTAGTGGACAGCCACATAAGGCTTTTAGATCGGAAGAG15.263157894736842No Hit
AATGGAGAATTTGGCTCAGGTAATGGATTTTGACACCATAATCACAAAAC15.263157894736842No Hit
CAGAAATCCAGCACAAGCTATAATAACCAAACATTGTGTCTCGCACAGGA15.263157894736842No Hit
GGGTCTCTGTGATTGGTTTACCGAATCATTTTGACATTCATCCATATATC15.263157894736842No Hit
AGACTATAATATTTTATTATTTAAAACAGAATATTACTGTAGAGCCAGAC15.263157894736842No Hit
CTCAGAAAACCAAAAGAGACTAGAAGTTTAAAGATCAATTTCTCAAGCTG15.263157894736842No Hit
GCAGCGCGCGCCCAGCCCCGCTTATACAGATAGGAAGAGCTTAGTGTAAG15.263157894736842No Hit
GAAATCTGCATTTCAACAGTAGTCCACAGGTGAATCTAAGCAGACCCTGG15.263157894736842No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)