FastQCFastQC Report
Sun 3 Mar 2019
41_S5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename41_S5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGAAGCGGGGCTGGGCGCGCGCCGCAGATCGGAAGAGCACACGTCTGA210.526315789473683Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCAGGAGGCTGAGGCAGGAGAATTGCCTGAACTCGGGAGGTGGAGGTTG15.263157894736842No Hit
AAAAGCCTTATGTGGCTGTCCACTACATTAAGAAAAATAGATCGGAAGAG15.263157894736842No Hit
CCAGTATTGCTAGTTAGAAGAAATTTGATAGTTAAAAAAAAAAAAAAAAA15.263157894736842No Hit
GTATTTTAATTTAAATTTTATACATTAAAAGGTACAATATCCTCTTTCCA15.263157894736842No Hit
GCCACACCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCAT15.263157894736842No Hit
GAAAAGTGACAGTTGGAATATGGTCACTGGCTGTGGTGAGCCCTTAAATA15.263157894736842No Hit
CTCTCAGCCGGGCGCGCCCCCCTCTCCCCCCCGCCACCCGACGCGTGACC15.263157894736842No Hit
CTCACACCTGTAATCCTAGCACTTTGGCCTAGCACCGCTTGAGGCCAGGA15.263157894736842No Hit
GGACCCCGCCCTTCGGGGAGGGGCCCGGCGGATGCCTCCTTTGCCGGAGC15.263157894736842No Hit
TACTTGTTCACTTATTAAGTAAAATTTTAAATTAACCGATTATGTACAGG15.263157894736842No Hit
CCGACCTGGGAGTGACCCACATGTCTCTTTGTCCCTTGCGCAGTCCTCCA15.263157894736842No Hit
GCAGGCTTCAGCGGGATCCACTGTGAGAACAACACGCCTGACTGCACAGA15.263157894736842No Hit
CAGATTTTTTTAAAAAAGATATTTCATTGCAAACAGTGGATGGAAGTAGG15.263157894736842No Hit
CTGCAATTCACATTAATTCTCGCAGCTAGCTGCGTTCTTCATCGACGCAC15.263157894736842No Hit
GAAAAAATGACTAAGTAATAGGGCCACTGACAACACATACAGCAACTTTT15.263157894736842No Hit
CTCCAACTAAAAAAAAAAAAAAAAAAGGCCCGGCCGCGGGGACGCGAATC15.263157894736842No Hit
CACAAGAGGTATTCTGTTGAAATGAATGATAGTATATCACTATACCATTG15.263157894736842No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)