FastQCFastQC Report
Sun 3 Mar 2019
2_S2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2_S2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44540711
Sequences flagged as poor quality0
Sequence length76
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT4533311.0177902189302725TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT2102430.47202434644565955TruSeq Adapter, Index 6 (97% over 36bp)
CGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCT1581780.3551312865212233No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG1385320.3110233242572172TruSeq Adapter, Index 6 (97% over 36bp)
GGGACGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCA928020.20835320747349542No Hit
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA926420.20799398554728954No Hit
CGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCACCGC863810.19393718254744519No Hit
GGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGCGGGCGGGC794710.1784232856094282No Hit
CGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCC739410.16600767778493702No Hit
CGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCCTCGGAGG703610.15797008718608016No Hit
GTCCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGT673970.151315501003116No Hit
CGCGGGGCGGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGC658030.1477367525632898No Hit
GCGGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGCGGGCGG533830.11985215054155737No Hit
CGCGCGGCCACCTCGAGCGTTCGCGTTCAGGGCGGGGGCCCGGCCGGTGC524040.11765416138058504No Hit
CCCGAACCCACCGCGATCGCTCACACGGCCCGCGCGCACCCGCCAGAGGG518260.11635647217216627No Hit
CCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCTTCTC514850.11559088044193995No Hit
GGGAGAGCGAGCGGGGCCGTGCCCGGCGGCGCGGAGCGGCGCGGCGGAGG466930.10483218375207347No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)