FastQCFastQC Report
Wed 31 Jul 2019
2_S1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2_S1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48155972
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGG2373660.492910827342453No Hit
CGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCTGGGCCT1559130.32376669709833705No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA1535460.31885141888528384No Hit
CCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTC1202660.24974264874146868No Hit
GGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGT1111460.2308041876924424No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1091570.22667385885181596No Hit
GCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGA982430.20401000316222462No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA899480.18678472526730433No Hit
CCGCTACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACT830790.17252065849693574No Hit
CGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCC768330.15955030458112235No Hit
GGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGAT721870.14990248769145392No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC644750.13388785922543522No Hit
GGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGGG631410.13111769397988685No Hit
GTAACGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCG597410.1240573027993288No Hit
CGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAG590120.12254347186679151No Hit
GAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACT584820.121442881476881No Hit
CGCCCCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTCCCC571950.11877031575647565No Hit
GGGGGGGCGAGCCCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC570900.11855227426413488No Hit
CCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTCC570480.11846505766719857No Hit
CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTC567970.11794383467122209No Hit
CCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCC561840.1166708876730803No Hit
CGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACC522450.10849121683183967No Hit
CCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCTGGGCC498070.10342850103825128No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG493380.10245458237246254No Hit
GAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGTG483130.10032608209008843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)