FastQCFastQC Report
Sun 3 Mar 2019
29_S5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename29_S5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44357750
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG4637361.0454452716830769No Hit
GGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCAC3998220.9013577108847947No Hit
TTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTA3801760.8570678179123152No Hit
GGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGAT2796690.6304850899786395No Hit
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA2765290.6234062818785895No Hit
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCC2463090.5552783899093169No Hit
GTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATA2382680.5371507797397298No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT2166520.48841972372358833No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA1592730.35906465048385006No Hit
TGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTC1409760.3178159397174113No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC1361640.3069677790239586No Hit
TTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAA1334890.3009372657540114No Hit
CCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACAC1145220.2581781086732307No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC1035200.23337522755324608No Hit
TTCCGAGATCAGACGAGATCGGGCGCGTTCAGGGTGGTGGTGCATGGCCG1029510.23209247538479746No Hit
GGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCA1028050.23176333335211996No Hit
CCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG1020830.23013565836860528No Hit
CTGCAATTCACATTAATTCTCGCAGCTAGCTGCGTTCTTCATCGACGCAC987140.2225405932446979No Hit
TGCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTT834480.18812496125254324No Hit
GCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGC826240.1862673377256511No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC815900.18393629072709955No Hit
CGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCACAG807180.18197045612097096No Hit
GGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACC782150.17632769921828767No Hit
TTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGAC772080.17405752095180663No Hit
CCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAAC763740.17217735345007357No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC652700.14714452378671145No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG610570.13764674718623013No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT594540.13403294801923002No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG584410.1317492433678444No Hit
CCGGGCCCGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGTCCGCCC552570.12457124177849419No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG544190.12268205668682475No Hit
CCGCGGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTT530210.11953040900406355No Hit
GTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCC484680.10926613725899081No Hit
TGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAA484030.10911960142252482No Hit
TTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGG476520.10742654891197141No Hit
GGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGC464920.10481144783042423No Hit
ATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTG457750.10319504483432995No Hit
CTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGT452420.10199345097530871No Hit
GGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGA446860.10074000597415335No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)