FastQCFastQC Report
Sun 3 Mar 2019
29_S5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename29_S5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44357750
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG7060991.5918278091201652No Hit
TTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTA4016170.9054043543687406No Hit
GGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCAC3801470.8570024403852764No Hit
GGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGAT3020280.6808911633254617No Hit
GTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATA2824090.6366621390850528No Hit
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCC2598220.5857420631118575No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT2499540.5634956687388337No Hit
CCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACAC2209910.49820155440706526No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC1616770.3644842220356082No Hit
TGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTC1594120.3593780117341389No Hit
TTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAA1581940.35663215559851436No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA1533140.3456306958761434No Hit
TTCCGAGATCAGACGAGATCGGGCGCGTTCAGGGTGGTATGGCCGTTCTT1381900.31153518832672983No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC1194210.26922240194779945No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC1169500.2636517857646071No Hit
CGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCACAG1163760.262357761608738No Hit
CTGCAATTCACATTAATTCTCGCAGCTAGCTGCGTTCTTCATCGACGCAC1141940.2574386662984484No Hit
GGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCA1119910.25247222864099284No Hit
CCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG1034480.23321291093439137No Hit
TTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGG904980.2040184635153947No Hit
TTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGAC892270.20115312431311327No Hit
GCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGC884090.19930902717112567No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG858010.19342955853261268No Hit
TGCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTT816240.1840129402415587No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT803020.18103262676758852No Hit
CCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAAC768730.17330229779463566No Hit
GGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACC757010.17066014394327936No Hit
CCGCGGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTT755250.17026336998607908No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG726170.16370758210233838No Hit
ATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTG623530.1405684463256139No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG600440.13536304253484452No Hit
TGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAA559680.12617411838968387No Hit
CCGAGATCAGACGAGATCGGGCGCGTTCAGGGTGGTATGGCCGTTCTTAG559560.12614706561987477No Hit
GTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCC551850.12440892515963953No Hit
CGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCA550960.12420828378355529No Hit
CCGGGCCCGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGTCCGCCC515880.11629985740935914No Hit
CCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTCTCCCCGGG506030.1140792758875281No Hit
GGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGC495310.11166256178458106No Hit
GCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAG491890.11089155784502144No Hit
CTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCC474960.107074862904453No Hit
TACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGG468360.10558696056495202No Hit
CCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACCA467320.1053525032266064No Hit
GGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGA462970.1043718403210262No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA455450.10267653341298871No Hit
CTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGT452820.10208362687467241No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT450170.10148621154138793No Hit
CCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACC443840.10005917793395744No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)