FastQCFastQC Report
Sun 3 Mar 2019
27_S3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename27_S3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGGCACAAGCCCACGCCAAGGGCATGAGCCCACAGGGCAGCTGGGGA212.5No Hit
TGCAAATTATCACAAACATGCCACTCCACACCCAAATTCCTCTACAGAGA16.25No Hit
CAAGAATAAATACATATCTGAAAACAACCAACCATAAAGAAGTATACTAA16.25No Hit
TAAGACTTTAACCTGAATATCAAATTTAAGAGTCCAGACTAGTTACTGTT16.25No Hit
CACCAGGAATTCACACACCTCACAGTAAACATCAGACTTTGCTGGGACCT16.25No Hit
CAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGGAGTG16.25No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG16.25No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCCGGAGGTCACCATAT16.25No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG16.25No Hit
CGGTGGCCTGCAGGCTATAGGTGAGACCCAGAGCCAGGTAGCCCTTGGGG16.25No Hit
CAAGAATAAATACAAGTCTGTAAACAACCAACCATAAAGAAGGATACTAA16.25No Hit
CAAGAATAAAAACATATAAGAAAAAAACCAACCATAAAGAAGTATACTAA16.25No Hit
CGGCGGGGGGGCGGACGAGGAGGCGGGGGAGGGGACCGGCGTCCGGCCCC16.25No Hit
TGCTACCATGCCAAGCACTGCCAGGAGAACAAATGCCCGGTGCCGTTCTG16.25No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA16.25No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)