FastQCFastQC Report
Sun 3 Mar 2019
25_S1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename25_S1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90190376
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT42971874.764573772261467TruSeq Adapter, Index 27 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA5237350.5806994307241827TruSeq Adapter, Index 27 (97% over 39bp)
CGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCGGTGCCGC2014920.22340742874827355No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1778300.19717181354250035No Hit
GGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCAC1722030.19093278866028898No Hit
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG1629270.18064787755181327No Hit
CCGGGACACACGCGCGGGGCCTCACCGCCGCCGGCGGCACCGCGCGGCAC1521520.16870092658223312No Hit
CGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCGGTGCC1356560.15041072674982528No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG1235830.13702459783513932TruSeq Adapter, Index 27 (97% over 38bp)
CGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGG1232440.13664872624546992No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG1104770.12249311389942537No Hit
GGGGACGCCACGGGGACCCCTCGAGCGCGGCCGACCGCAGCCGGGACACA1049390.11635276916907408No Hit
CGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGG1025350.11368729630310001No Hit
GGGAAGGGGACGCCACGGGGACCCCTCGAGCGCGGCCGACCGCAGCCGGG984690.1091790547585698No Hit
GGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGGGGGGACGAACC947890.10509879679401714No Hit
GGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGGGGGGACGAACCGC940440.10427276630934547No Hit
GGGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGC919550.10195655465501108No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)