FastQCFastQC Report
Sun 3 Mar 2019
22_S8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename22_S8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29412212
Sequences flagged as poor quality0
Sequence length76
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGG2017880.6860687662662026No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1978170.6725675715923712No Hit
GGGGGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGC1693480.5757744436222614No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG1619710.5506930250604749No Hit
CGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGG1461450.4968854433661773No Hit
CGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCACCGC1344840.4572386463146668No Hit
GGGACGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCA1217660.4139981039168356No Hit
CGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCGGTGCCGC1144610.38916148163218733No Hit
CGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCCT1077870.36647022672079205No Hit
CGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGG1042120.3543154115712208No Hit
GGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCCTC1041840.3542202130190004No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC1030770.3504564702580003No Hit
CGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCT1020210.3468661248599731No Hit
CGACGCGCCGCGAGGCGAGCCGGGCGGGCGGGCGCGCGCGCGTACGCGCG907090.3084059097629243No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGCGGTTCGTGCCCCCCC826390.28096832703368246No Hit
GGGGGAGTCCCGTCGGGAGGGGCCCGGCCCCTCCCGCGCCTCCACCGCGG792020.2692827047486262No Hit
GGGAGCGGTCGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGCGGTTCG716690.2436708942530402No Hit
CGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCGGTGCC658860.22400899327123033No Hit
GGGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGC615380.2092260180907169No Hit
GGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCCTCG597960.20330330816328945No Hit
GGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGC591070.20096074378900844No Hit
CGCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAG577360.19629941467850157No Hit
CGCGAGGCGAGCCGGGCGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGC577150.19622801576433627No Hit
CTCCGAGGCCCCGCCCGTCCTCCTCGCCCTCCCCGCGCGTACGCGCGCGC573730.19506523344792973No Hit
CGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCCTCGGAGG554940.18867673060428097No Hit
GCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCGGTG543510.18479058970471177No Hit
CCGCCCTCCTCTTGCCGCCGCGCCCGCCCCTTCCCCCTCCCCCCGCGGGC532370.18100304730565658No Hit
GGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGGGGG531550.1807242515455825No Hit
CGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCC520450.17695030893970165No Hit
GAGCGGTCGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGCGGTTCGTG516880.1757365273988913No Hit
GGGGAGTCCCGTCGGGAGGGGCCCGGCCCCTCCCGCGCCTCCACCGCGGA510670.1736251595085742No Hit
CCGGCGTCCGGCCGACCGCCGCTCCCGCGCCCCTCCTCCTCCCCGCCGCC493280.1677126494260275No Hit
GCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCGGTGCCG490950.1669204614736219No Hit
CGGCGGGGAAGGGGACGCCACGGGGACCCCTCGAGCGCGGCCGACCGCAG489780.16652266752327233No Hit
GGGCGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGC478790.16278612434862091No Hit
GGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGCGGGCGGGC478670.16274532496909785No Hit
GGGAGGGCGAGGAGGACGGGCGGGGCCTCGGAGGAGGGGCGGCGGGGAGG472560.16066795656171662No Hit
GCGGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGCGGGCGG460650.15661861814405528No Hit
CGGGGGTTCCCGCCCCCACGGCGCGGGGCGCACGCCACACGCGCGGCAGG460620.15660841829917452No Hit
GGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCGCGGGAGCGGCG455010.15470104730647255No Hit
CGCGCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGT453180.15407885676874625No Hit
CGCGGGGCGGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGC452680.15390885935406695No Hit
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA445750.1515526951866116No Hit
GGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGG443130.15066190873369198No Hit
GTCGGGAGGGGCCCGGCCCCTCCCGCGCCTCCACCGCGGACTCCGCTCCC438190.14898233427666033No Hit
GTCCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGT432340.14699336452491232No Hit
CCGAGGCCCCGCCCGTCCTCCTCGCCCTCCCCGCGCGTACGCGCGCGCGC426980.14517099223955002No Hit
CCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCTTCTC421210.14320922207415068No Hit
CGGCGGGGAAGGGGACGCCACGGGGACCCCTCGAGCGCGGCCGACCGCGG419350.14257683169154364No Hit
CCGGGACACACGCGCGGGGCCTCACCGCCGCCGGCGGCACCGCGCGGCAC401180.13639912564209722No Hit
GGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCG398270.1354097406886636No Hit
CGAGGCCCCGCCCGTCCTCCTCGCCCTCCCCGCGCGTACGCGCGCGCGCC393600.13382196483555878No Hit
CCGGGGGTTCCCGCCCCCACGGCGCGGGGCGCACGCCACACGCGCGGCAG387940.1318975941013889No Hit
CGGCGGTGGTGGGGGGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCC387810.13185339477357227No Hit
GGCGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCG383970.13054781462883513No Hit
GGGGACGCCACGGGGACCCCTCGAGCGCGGCCGACCGCAGCCGGGACACA383910.1305274149390736No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG380240.12927963391532743No Hit
GGGGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCG367460.12493449999612406No Hit
GTCCGCCCTCCTCTTGCCGCCGCGCCCGCCCCTTCCCCCTCCCCCCGCGG367400.12491410030636253No Hit
CCGCGGGGGAGTCCCGTCGGGAGGGGCCCGGCCCCTCCCGCGCCTCCACC365310.124203511113003No Hit
CGCGCCGCGAGGCGAGCCGGGCGGGCGGGCGCGCGCGCGTACGCGCGGGG364210.1238295168007085No Hit
GGGGACGCCACGGGGACCCCTCGAGCGCGGCCGACCGCGGCCGGGACACA360390.12253073655255851No Hit
CGGGGGAGTCCCGTCGGGAGGGGCCCGGCCCCTCCCGCGCCTCCACCGCG355950.12102115951020617No Hit
GTCGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGCGGTTCGTGCCCCC342750.11653322776267218No Hit
GGGGGTTCCCGCCCCCACGGCGCGGGGCGCACGCCACACGCGCGGCAGGC341640.1161558335020841No Hit
GGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGGGGGGACGAACC340770.11586003800054209No Hit
CCGACGGCCGCCGCGGCGTCGGCGGCGGCGCGGCCCCGGCCGGGGAGCGG340200.11566624094780766No Hit
CGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCGCC339790.11552684306777063No Hit
GGGCGGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGCGGGC337210.11464965640802535No Hit
CGGCGTCCGGCCGACCGCCGCTCCCGCGCCCCTCCTCCTCCCCGCCGCCC335250.11398326654248243No Hit
GGTCGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGCGGTTCGTGCCCC335030.11390846768002352No Hit
CGGGGAGCGGTCGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGCGGTT317920.10809115614969728No Hit
CTCCTCCGAGGCCCCGCCCGTCCTCCTCGCCCTCCCCGCGCGTACGCGCG310420.1055411949295075No Hit
GGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCACCGCC304410.10349782600506212No Hit
GGCCGACGCGCCGCGAGGCGAGCCGGGCGGGCGGGCGCGCGCGCGTACGC300700.10223644518814158No Hit
CGGGCGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGG294770.10022027585004487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)