FastQCFastQC Report
Sun 3 Mar 2019
22_S8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename22_S8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29412212
Sequences flagged as poor quality0
Sequence length76
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT4481691.5237514267883012TruSeq Adapter, Index 6 (97% over 36bp)
CGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGG2186040.7432422967711507No Hit
CCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCG1953240.6640915004964604No Hit
CGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGG1630520.5543683691658416No Hit
GCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGC1246680.4238647538648232No Hit
GGGACGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCA1211010.4117371383016007No Hit
CGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCT1184650.40277487459970707No Hit
CGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCACCGC1172180.39853513907760496No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA925690.31472981358899493No Hit
CGACGCGCCGCGAGGCGAGCCGGGCGGGCGGGCGCGCGCGCGTACGCGCG854390.29048818225572426No Hit
CGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCGGTGCCGC852550.28986259176970436No Hit
CGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCCT812230.27615400024996417No Hit
GGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCCTC806660.2742602290504366No Hit
CGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGG699040.2376699855148603No Hit
CGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCCTCGGAGG698770.23757818691093344No Hit
CGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCGGTGCC694800.2362284074383797No Hit
GGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCCTCG682850.232165469227544No Hit
GCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCGGTG678530.23069669156471467No Hit
CTCCGAGGCCCCGCCCGTCCTCCTCGCCCTCCCCGCGCGTACGCGCGCGC662490.22524317450180217No Hit
CGCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAG661020.224743382102645No Hit
CCGGGACACACGCGCGGGGCCTCACCGCCGCCGGCGGCACCGCGCGGCAC642650.21849767708732684No Hit
GGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGGGGG619530.2106369966325552No Hit
GGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGCGGGCGGGC617250.20986180842161753No Hit
GGGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGC582600.19808098758434084No Hit
CCGCCCTCCTCTTGCCGCCGCGCCCGCCCCTTCCCCCTCCCCCCGCGGGC563470.19157688649871013No Hit
CGCGCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGT542860.18456959306562865No Hit
CCGACGGCCGCCGCGGCGTCGGCGGCGGCGCGGCCCCGGCCGGGGAGCGG539100.1832912125072402No Hit
CGGCGGGGAAGGGGACGCCACGGGGACCCCTCGAGCGCGGCCGACCGCAG536390.18236982651967829No Hit
GTCGGGAGGGGCCCGGCCCCTCCCGCGCCTCCACCGCGGACTCCGCTCCC529430.1800034625073422No Hit
CGCGAGGCGAGCCGGGCGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGC527190.17924187408957887No Hit
CGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCC513420.17456014528931044No Hit
GGGAGGGCGAGGAGGACGGGCGGGGCCTCGGAGGAGGGGCGGCGGGGAGG502830.17095960004640248No Hit
CGCGGGGCGGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGC492300.16737945449325606No Hit
GTCCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGT489010.16626087150466615No Hit
CCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCTTCTC487450.16573047957086667No Hit
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA479230.1629357220735387No Hit
CCGAGGCCCCGCCCGTCCTCCTCGCCCTCCCCGCGCGTACGCGCGCGCGC479120.16289832264230925No Hit
CGGCGGGGAAGGGGACGCCACGGGGACCCCTCGAGCGCGGCCGACCGCGG474790.16142614503118635No Hit
GGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCG466140.15848518975723416No Hit
GCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGG458660.15594202843363159No Hit
GCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCGGTGCCG447890.1522802841214391No Hit
GCGGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGCGGGCGG442220.15035251343897563No Hit
GGGGAACGGGGGGCGGACGGGGCCGGGGGGGTAGGGCGGGGGGACGAACC420050.1428148280720947No Hit
GGGAGCGGTCGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGCGGTTCG419280.14255303205348852No Hit
CGAGGCCCCGCCCGTCCTCCTCGCCCTCCCCGCGCGTACGCGCGCGCGCC414560.14094825645891576No Hit
GGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCGCGGGAGCGGCG379110.12889543975815215No Hit
GTCCGCCCTCCTCTTGCCGCCGCGCCCGCCCCTTCCCCCTCCCCCCGCGG378690.12875264192982153No Hit
GGGAAGGGGACGCCACGGGGACCCCTCGAGCGCGGCCGACCGCAGCCGGG377470.12833784823800398No Hit
CGCGGGGTGGGGAGGGAGCGAGCGGCGCGCGCGGGGTGGGGCGGGGGAGG371670.1263658782277239No Hit
CTCCTCCGAGGCCCCGCCCGTCCTCCTCGCCCTCCCCGCGCGTACGCGCG370070.1258218865007501No Hit
GGGGAGTCCCGTCGGGAGGGGCCCGGCCCCTCCCGCGCCTCCACCGCGGA361320.12284693174386203No Hit
GGGAAGGGGACGCCACGGGGACCCCTCGAGCGCGGCCGACCGCGGCCGGG345800.11757021199221601No Hit
CGCGCCGCGAGGCGAGCCGGGCGGGCGGGCGCGCGCGCGTACGCGCGGGG340540.1157818391897896No Hit
GCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCTTCT338550.11510524947936593No Hit
GGGGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCG338340.1150338505652006No Hit
GCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCGGTGCCGCCGGCGGCG336890.11454085806263058No Hit
CCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCC335970.11422806281962065No Hit
CGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCTTCTCGCT333710.11345967450527013No Hit
GGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGC330730.11244648991378138No Hit
CGACGGCCGCCGCGGCGTCGGCGGCGGCGCGGCCCCGGCCGGGGAGCGGA330440.11234789141326738No Hit
GGGGACGCCACGGGGACCCCTCGAGCGCGGCCGACCGCAGCCGGGACACA329410.111997696739028No Hit
CCGGGGGTTCCCGCCCCCACGGCGCGGGGCGCACGCCACACGCGCGGCAG328210.11158970294379764No Hit
CCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGCGGGCGGGCGCGCGC327110.11121570863150314No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323730.11006652610827095No Hit
CGGCGGTGGTGGGGGGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCC322500.10964833246815983No Hit
CGCCGCCCCTCCTCCGAGGCCCCGCCCGTCCTCCTCGCCCTCCCCGCGCG317690.10801295733894478No Hit
GGGGACGCCACGGGGACCCCTCGAGCGCGGCCGACCGCGGCCGGGACACA316910.10774776137204506No Hit
CGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGTGGGGAGGGAGC316420.10758116390565933No Hit
CCGCGGGGGAGTCCCGTCGGGAGGGGCCCGGCCCCTCCCGCGCCTCCACC314080.10678557600496011No Hit
GGGCGGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGCGGGC305630.10391261969687965No Hit
GGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCACCGCC302240.10276003722535387No Hit
GGGGAAGGGGACGCCACGGGGACCCCTCGAGCGCGGCCGACCGCAGCCGG301920.10265123887995911No Hit
CCGGCGGGGAAGGGGACGCCACGGGGACCCCTCGAGCGCGGCCGACCGCA295300.10040047310960495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)