FastQCFastQC Report
Sun 3 Mar 2019
20_S6_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20_S6_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55454311
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1100840.19851297043434551No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG980110.1767418947825355No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATC948500.1710417067484618Illumina Single End PCR Primer 1 (96% over 33bp)
CGGGAACGTATTCACCGCAGCGTTGCTGATCTGCGATTACTAGCGACTCC651140.11741918495750492No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC603440.10881750924648581No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAA569450.10268813907001748No Hit
CCTCGATCTATTAGTACCAGTCAGCTCCAAACCTCACGGCTCTTCCACAC562910.10150878982158845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)