FastQCFastQC Report
Sun 3 Mar 2019
20_S6_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20_S6_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55454311
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT21986073.9647179098483436TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTAT1490240.2687329394463128TruSeq Adapter, Index 3 (97% over 36bp)
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG937260.16901481293311896No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTA810440.1461455359169461TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTTT789470.14236404451946036TruSeq Adapter, Index 3 (97% over 36bp)
CGGGAACGTATTCACCGCAGCGTTGCTGATCTGCGATTACTAGCGACTCC617730.11139440538716638No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTGGTAT590210.106431761454939TruSeq Adapter, Index 3 (97% over 36bp)
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAA586190.1057068403572808No Hit
CCTCGATCTATTAGTACCAGTCAGCTCCAAACCTCACGGCTCTTCCACAC576360.10393420991201208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)