FastQCFastQC Report
Sun 3 Mar 2019
19_S7_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename19_S7_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18545100
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT2299211.2397937999795094TruSeq Adapter, Index 19 (97% over 38bp)
CGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCT927360.5000566187294757No Hit
CGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCACCGC835260.45039390458935247No Hit
GGGACGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCA705140.38022981811907186No Hit
CGACGCGCCGCGAGGCGAGCCGGGCGGGCGGGCGCGCGCGCGTACGCGCG501340.2703355603366927No Hit
CGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCCT363110.19579835104690727No Hit
CCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCTTCTC361850.19511892629319874No Hit
GTCCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGT347440.18734867970515123No Hit
GGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCCTC334750.18050590182851534No Hit
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA330570.17825193716938706No Hit
CGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCC300040.16178936754183046No Hit
CGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGG298440.16092660594981964No Hit
GCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCTTCT274590.14806606596890823No Hit
GGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCCTCG264880.14283018155739252No Hit
CGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCCTCGGAGG262850.14173555278752878No Hit
CCGGGGGTTCCCGCCCCCACGGCGCGGGGCGCACGCCACACGCGCGGCAG246030.13266577155151496No Hit
CCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCC245600.13243390437366206No Hit
CGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCTTCTCGCT229990.1240165865916064No Hit
GGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCACCGCC225770.12174105289267784No Hit
CGGGGGTTCCCGCCCCCACGGCGCGGGGCGCACGCCACACGCGCGGCAGG216620.11680713503836593No Hit
CGCGAGGCGAGCCGGGCGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGC212220.11443454066033616No Hit
CGCGCCGCGAGGCGAGCCGGGCGGGCGGGCGCGCGCGCGTACGCGCGGGG200940.10835207143665981No Hit
GGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGC190190.10255539199033707No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)