FastQCFastQC Report
Sun 3 Mar 2019
18_S6_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename18_S6_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43107305
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGCGCCGCGAGGCGAGCCGGGCGGGCGGGCGCGCGCGCGTACGCGCG855610.19848376046704844No Hit
CGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCT728320.1689551225714528No Hit
CCGGCGTCCGGCCGACCGCCGCTCCCGCGCCCCTCCTCCTCCCCGCCGCC684560.15880371087916537No Hit
CGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGG658730.15281168702149206No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATC650600.15092569577244505Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG646340.14993746419545365No Hit
GGGACGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCA631530.1465018516003262No Hit
CGCGAGGCGAGCCGGGCGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGC558780.1296253616411418No Hit
CGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCCT534690.12403698166702835No Hit
CGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCACCGC491720.11406883357704686No Hit
GGGGGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGC465050.10788194715489637No Hit
CTCCGAGGCCCCGCCCGTCCTCCTCGCCCTCCCCGCGCGTACGCGCGCGC461170.10698186769040653No Hit
GTCCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGT457240.10607018926374545No Hit
GGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCCTC433620.10059083953404187No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)