FastQCFastQC Report
Sun 3 Mar 2019
17_S5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename17_S5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27329588
Sequences flagged as poor quality0
Sequence length76
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAGAGAGAGAG788390.28847489395010273No Hit
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA586130.21446719211427556No Hit
CGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCT549720.20114463489167858No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT473820.17337253675393863TruSeq Adapter, Index 13 (97% over 38bp)
CGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGCGGGGAGGGAGC469310.1717223106327106No Hit
CGACGCGCCGCGAGGCGAGCCGGGCGGGCGGGCGCGCGCGCGTACGCGCG432220.15815093882864242No Hit
CGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCC365380.13369392908520977No Hit
CGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAGAGAGAG362340.13258158154451505No Hit
CGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCACCGC339660.12428288344485837No Hit
GGGAGGACGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGCGGGG337450.123474236055077No Hit
GGGACGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCA317220.11607200225630916No Hit
GGACGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGCGGGGAGGG298780.10932473625288461No Hit
GTCCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGT282880.10350686589201419No Hit
CGGGGAGCGGGGGAGAGAGAGAGAGAGAGGGCGCGGGGTGGGGAGGGAGC281510.10300557769110899No Hit
CGCGGGGCGGGCTCCCGGCCCCGGCCGACGCGCCGCGAGGCGAGCCGGGC280930.10279335348926592No Hit
GGGAGAGAGAGAGAGAGAGGGCGCGGGGCGGGGAGGGAGCGAGCGGCGCG280410.10260308351519971No Hit
GGCGGTTTGAGTGAGACGAGACGAGACGCGCCCCTCCCACGCGGGGAAGG280320.1025701521735344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)