FastQCFastQC Report
Sun 3 Mar 2019
12_S10_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename12_S10_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGGGCTCCCTACACGGAGAAAAGAGATCGGAAGAGCGTCGTGTAGGG12.0Illumina Single End PCR Primer 1 (100% over 24bp)
AGGCACCAATCCATCTCTGGAATGTTCTCTGGATGTCAAGAGTAGGTAAG12.0No Hit
CGGGGTTAAGCCCCGGCCTTTCACAGCAGACGCGACAAACCGCCTACAAG12.0No Hit
CCCCAGGTGGTATACTTAATGCTTTCGCTTGTCTGCTTACACTCTATCGC12.0No Hit
CGGGAACGTATTCACCGCGCCATGGCTGATGCGCGATTACTAGCGAATCC12.0No Hit
CCCTCCTTCAGTGATAGCTTATAAATAGAGGCCACCTTTCATCCATCCTC12.0No Hit
CTCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTA12.0Illumina Single End PCR Primer 1 (97% over 35bp)
CAAGCATTAACCCACTTCAGCCCCATAAAGTTGTTCCACCCCATGCAGCC12.0No Hit
AATAACAAGACAACTTACTCTAAAAATCTATATCACAGAAAAACCACCAT12.0No Hit
AACCCAACCCCTCACTTCAATTTCAATCACTTAAACCTGGGAACTCTGGA12.0No Hit
AGAGCATTAAGTCAAGTTATCTGATATATCTTCTGACTCTGTTAGCCAGA12.0No Hit
CTAACTCGTCATCACATATCAGATTTTAGACATACGGATTTGCATATAGC12.0No Hit
AGCCCAACCCCTCTCTTCAGTTTCAATCAGTTAAACCTGGGCACTCTGGA12.0No Hit
CCCTACTTGTCGTTAGCTTAGTACCATGACCTGGACTTCGAGTACGGGGC12.0No Hit
CCTAGATCTGCTTCCCAACACCTCCTCAATAGCAGGAGTAAATATTGAGA12.0No Hit
GCGCCATCCCACTACTGATCAGCACGGGAGTTTAGATCGGAAGAGCGTCG12.0No Hit
CGTATGCCGTCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG12.0Illumina Single End PCR Primer 1 (100% over 35bp)
GGATAGGGGTGAAAGGCCAATCAAACTCTGTGATAGCTGGTTCTCCCCGA12.0No Hit
AAACAATTCCACTACCTTCAATCAATCTAACTATTCGCAAGAATGCCTAT12.0No Hit
TGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGATAAACCACGAG12.0No Hit
CCACCATCACACTACTTATCAGCACGCTACATTAGAAATGAAGAGCGTCC12.0No Hit
GCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTT12.0No Hit
CAGGAACGAATTCACAGAGCCATTGCTGATGAGAGAATACTATAGAATCC12.0No Hit
CTCGAGATCGGAAGAGCGTCGTGTATGGAAATATTGTAGGATATGGTGTA12.0Illumina Single End PCR Primer 1 (96% over 27bp)
CCTATCCCGTCATCAAGATCGGAAGAGCCTCGTTTACGGAAATACTGAAA12.0No Hit
CAAGCAGAACACTGCATATCAAGTACATCCCTTTAGTCAGAGTAACTGCC12.0No Hit
AATCAATTCCGCTACATTCAATCATTCTAACTATTCCAAAGAATGCCTAT12.0No Hit
AGGGCATTGAGTCAAGGTATCTGATATCTCTTCTGAGGCTGTGAGCCAGA12.0No Hit
CGGGAACGTATTCACCGCGCCATGGATGATGAGCGATTACTAGCGAATCC12.0No Hit
AAAATTTGGCATTAGATCGGAAGAGCGTCGATTAGGGAAAGATTGAATGA12.0No Hit
CGGGTTGAAAGCTTGCTTTCAATGCCGGCGACCGGCGCACGGGTGCGTAA12.0No Hit
CAATACCCCACTACAATACTACACATCTATTGCCCTCTCCTGAAACCTGG12.0No Hit
CATATGCCGTCTTCTAGATCGGAAGAGCGTCGTTTAGGGAAATAGTGTAG12.0Illumina Single End PCR Primer 1 (96% over 27bp)
CGAGGTTAAGCGGAAAACGTGGAGCCGAAGCGAAAGCGAGTCTGAGATCG12.0No Hit
AATCAATTCCACTACCGTCAATCAATCTAACTATTCGCAAGAATGCCTTT12.0No Hit
GATCAAGTGTAGTATCTGTTCTTATAATTTTAATATCTGATACGTCCTCT12.0No Hit
GGAAGGGCTCCCTACACGGAGACAAGAGATCGGAAGAGCGTCGTGTAGGG12.0Illumina Single End PCR Primer 1 (100% over 24bp)
CGGCGCTCTGTCCGGCGCGTGACTCCCTCCGCCGCGAGTCGGCTCTCCGC12.0No Hit
CAACAAAATAAAGAAAGAACGGAAGAGAGTCTTTTACTGAAATACAGTAG12.0No Hit
CAGTCCCTATACTCCCAGCTACTACTAACGCTGAGTCTGGAGTATCGCAT12.0No Hit
AAGGCATAAAAACAAGGTATCTGATATCTCTTCTGAGGCTGTGAGCCAGA12.0No Hit
CGTATGCCGTCTTCTAGATCGGAAGAGAGTCTTTTAGTGAAATAGTGTAT12.0No Hit
ATTCAATTACACTACCTTCAATAATTCTAACTATTCCCAAGAATGCCTTT12.0No Hit
CAAGAGTTGAACACATTGAAGGGACAGTCTCACAAATGTTCTTATTGATC12.0No Hit
CGGAGGGGTGCCTTCGGGAGCCGTAACACAGGTGCTGAGATCGTAAGAGC12.0No Hit
GCAGAAGGGCGAGCTGCGCAAGCGGCTGAGATCGGAAGAGCGTAGTGAAG12.0No Hit
CTGGCATTAACCCACTTCAGCCCCATAAAGTTGTTCCACCCCATCCAGCC12.0No Hit
CGGCCACCTACTCTCCCGCATTGCATTGCAGTACCATCGGCGCAAACGGG12.0No Hit
GAAGAGTTGAAGACATTGAAGGGCCAGGCTCCCCAATGTTCTTCTTGATC12.0No Hit
GCCCTCCTCATAGTCTAACCTATGGGTTACATTGCATAACCACTGCCGGG12.0No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)