FastQCFastQC Report
Sun 3 Mar 2019
12_S10_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename12_S10_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA48.0Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGA48.0No Hit
GGCTCACAGCCTCAGAAGAGATATCAGATACCTTGACTCAATGCCCTAGA36.0No Hit
ATGCAAGTTGCATCCAATCTTGAAATCCGGTCCCAGTTCGGACTGGGGTC36.0No Hit
CTTGTCTCCGTGTAGGGAGCCCTTCCAGATCGGAAGAGCACACGTCTGAA24.0Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACTCCCGTGCTGATCAGTAGTGGGATCGCGCAGATCGGAAGAGCACAC24.0No Hit
GCTACCTCTCTTCCTCGTGGTTTATCGGTGCTCTACACGTTCAGAGAAAC24.0No Hit
CCCGGACGTAAGGGCCGTGCTGATTTGACGTCATCCCCACCTTCCTCACA24.0No Hit
TGAGGGACAGGTCGTGGCTCCGTCATCCACAGACATAGTTCAGGCACCGC24.0No Hit
CGGAGGCCTGCAGAAGTCAGTGACTTGCCCTAATACTTCAGCTTTGATCA24.0No Hit
CGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCT24.0TruSeq Adapter, Index 1 (97% over 36bp)
CCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAAC24.0No Hit
GGGAGAGAGAGGTTTGCAGCCTGCAGGAAGCTGGCCAGCCAGACCTGCCG12.0No Hit
TTGGAAACGACTGCTAATACCGCATACGATCCAATCTCGGCATCGAGGAT12.0No Hit
AATGCCAAATTTTAGATCGTAAGAGCACACGTCTGAACTCCAGTCACAGC12.0TruSeq Adapter, Index 13 (97% over 35bp)
CAGCCGCTTGCGCAGCTCGCCCTTCTGCAGATCGGAAGAGCACACGTCTG12.0Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGACTCGCTTTCGCTTCGGCTCCACGTTTTCCGCTTAACCTCGAGATCG12.0No Hit
CGAAAAGGGGCTTTGTGGGTACAAGGCCAGTGGTGAGTACATTTGACCTG12.0No Hit
GGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGG12.0No Hit
AGCTATAGGCAAATCCGTAGCTCTAAAAGCTGAGATGTGATGACGAGTTA12.0No Hit
ATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCGGATAG12.0No Hit
CAGCTGTCAACGTTTTGAGCAACAGGTCCCAGGAGGACTTTCAGCTGGGG12.0No Hit
TGTCTCACTGCTACGCTCTCACTTACCGGCATTCGGAGTTTAGCTGACGT12.0No Hit
TTCACTACTCTGTCCAGATCGGAGGAGCACACGTCTGAACTCCAGTCACA12.0Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CGAAGAAGGACGCGGTAAGCTGCGATAAGCTACGGAGAGGTGCAAGCAAC12.0No Hit
CAGAGACAGAGGAACAAGCTGGGAAGCTTGGCGATACAGGGTGATAGCCC12.0No Hit
TGTAAACCTCTGTTAGCAGGGAAGAAGAGAGATTGACGGTACCTGCAGAG12.0No Hit
ACCCTGGTAGTCCACACAGTAAACGATGTATACTCGCGGTTTGCGATAGA12.0No Hit
CCCACCTCTTCCATTCCGAACAGAGAAGTTAAGCCCGTTTGCGCCGATGG12.0No Hit
GATTCTCGTCCATCCTCCGACCCGGTCCCGCTCCGGGAGACCGGCGCGCC12.0No Hit
TGCAGGTTTGATACGCGTTACGCACCCGTGCGCCGGTCGCCGGCATTGAA12.0No Hit
CAGCACCTGTGTTACGGCTCCCGAAGGCACCCCTCCGAGATCGGAAGAGC12.0No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)