FastQCFastQC Report
Sun 3 Mar 2019
10_S8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename10_S8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38930485
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGGGCGTGCGGCGCCCGTCCCGCCCCCGGCCCGTGCCCCTCCCTCCG1334170.34270572277740696No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT935810.2403797435351756TruSeq Adapter, Index 6 (97% over 36bp)
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA924970.23759529325154827No Hit
CGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCT891140.22890544518004335No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG676110.1736710960574984No Hit
CGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCACCGC664830.17077362380663894No Hit
CGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCC662010.17004925574392407No Hit
CGACGCGCCGCGAGGCGAGCCGGGCGGGCGGGCGCGCGCGCGTACGCGCG650020.16696940713684919No Hit
GGGAGAGCGAGCGGGGCCGTGCCCGGCGGCGCGGAGCGGCGCGGCGGAGG608290.1562503010173133No Hit
CGGCGGGGTCCTCTGACGCGGCAGACAGCCCTCGCTGTCGCCTCCAGTGG545440.14010614047063633No Hit
CCGGGGGTTCCCGCCCCCACGGCGCGGGGCGCACGCCACACGCGCGGCAG538670.13836714338390593No Hit
GGGACGGCGAGGTCGGGCCGGGGTCCGCACCCCACGCCTTCCCACACGCA485370.12467607326238037No Hit
CGGGGGTTCCCGCCCCCACGGCGCGGGGCGCACGCCACACGCGCGGCAGG476070.1222871998640654No Hit
CCCGAACCCACCGCGATCGCTCACACGGCCCGCGCGCACCCGCCAGAGGG430130.11048667901260413No Hit
CCGCCCTCCTCTTGCCGCCGCGCCCGCCCCTTCCCCCTCCCCCCGCGGGC422190.10844714624027932No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)