FastQCFastQC Report
Tue 25 Mar 2025
SH_dif_9_S14_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH_dif_9_S14_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42629906
Sequences flagged as poor quality0
Sequence length101
%GC69

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8976022.1055687995183474No Hit
GGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGG1987970.46633225041594034No Hit
GGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCAC1543330.36202988578018447No Hit
CGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC1429690.33537254339711653No Hit
GGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGAT1326380.31113838252423076No Hit
CGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTT1165800.2734699907618844No Hit
CGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCG1109520.26026799120786237No Hit
GGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCC998930.23432610899963044No Hit
GTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCG846090.19847334404162184No Hit
GGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACC843620.197893938588558No Hit
CCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGAGGGCGCGCGGGT816630.19156270248402613No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA782340.18351905350201805No Hit
CGGCGGTCGGCGGGCGGCGGGGCGGGGCGGTTCGTCCCCCCGCCCTACCC768270.18021855361351255No Hit
GGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCG720560.16902687986222628No Hit
CGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTC697870.16370432531565984No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGAC690330.1619356139326228No Hit
CCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTC668790.1568828230585355No Hit
CTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTC653590.1533172510396809No Hit
GGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGA634160.14875941786031618No Hit
GGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGC621470.14578263437878564No Hit
GGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCT613300.1438661394186513No Hit
ATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGG600130.14077675892599903No Hit
GGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCC578160.13562309989611518No Hit
GCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGG576610.13525950538103462No Hit
GGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGA562440.13193554778187874No Hit
CTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGC554690.13011757520647593No Hit
GGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTC554330.13003312744813467No Hit
GCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGC535530.12562307784586718No Hit
CCCGGCCCCGCGTCCTCCCTCGGGAGGGCGCGCGGGTCGGGGCGGCGGCG530990.12455809778234087No Hit
CGCCCCCCGGCCCCGCGTCCTCCCTCGGGAGGGCGCGCGGGTCGGGGCGG522510.12256888391919044No Hit
CTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGT511330.11994631186848032No Hit
CGCCGCCCCTCCTCCTCCTCCCCGGAGGGGGCGGGCTCCGGCGGGTGCGG507330.11900800344246595No Hit
GGGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTT480110.11262281460343825No Hit
CGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCA478730.1122990981964633No Hit
CGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCG476730.11182994398345612No Hit
GAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCC474800.1113772101679042No Hit
GGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCC468840.10997913061314281No Hit
GGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGGG451880.10600070288684194No Hit
GTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGG451490.10590921781530552No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA430990.10110038713198194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAAT136950.042.8036421
CGGAATC162750.036.7114032
ATCAGCG173600.035.9413456
TCAGCGG191450.032.76867
AATCAGC207600.030.0089045
GGAATCA247500.027.162243
GAATCAG269550.025.1711084
CAGCGGG322500.019.6513888
ATACCCG85100.019.5684838
CGCGAAG810250.019.4084451
GCGAAGC891700.018.0669652
CGAAGCG902450.017.5231723
CAGTCGA183900.017.146254
TAGCCGA41400.016.4116974
GATTCAA869500.016.4043455
GAAGCGG994050.016.3031854
AGTCGAG195900.016.2669545
AAGCGGG987400.016.2531095
GGATTCA889650.016.1349474
CCGCGCA446150.016.0361921
Produced by FastQC (version 0.11.4)