FastQCFastQC Report
Tue 25 Mar 2025
SH_dif_4_S2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH_dif_4_S2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40814210
Sequences flagged as poor quality0
Sequence length101
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGT1729490.4237470234018985No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA1420500.348040547642598No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC1362130.33373915604393667No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC1113250.27276039399023033No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1101930.26998685016811547No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1078800.2643197062983701No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA995200.24383664414918235No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA977120.2394068144403628No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG916640.22458844603386907No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG863770.21163462431344376No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT852140.2087851265527374No Hit
CCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGAGGGCGCGCGGGT825590.20228003923143434No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA784040.19209976133312393No Hit
GGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGA733780.1797854227730979No Hit
CTCCGTTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAAT712020.17445394630938588No Hit
TGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCC708130.1735008468864153No Hit
GGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGAT705210.17278540978742452No Hit
CCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCT689450.16892400955451545No Hit
CCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTC683830.16754703815166336No Hit
CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA662570.16233806804051826No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG657530.16110320400664377No Hit
CTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAG649710.15918720465249725No Hit
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGC626810.15357641370493266No Hit
GGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGG588820.14426838103689868No Hit
CTCGGATAGCCGGTCCCCCGCCTGTCCCCGCCGGCGGGCCGCCCCCCCCT587650.1439817161718921No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA586090.14359949635188332No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCTGTCCCCGCCGGCGGGC577560.1415095379770918No Hit
TGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGG576710.14130127717772806No Hit
CGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAA564140.1382214674741959No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT563320.13802055705598612No Hit
CGGGCGATGGCCTCCGTTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCA550130.13478883947527098No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT547990.1342645122862846No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG534660.1309984929268507No Hit
CTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAG526120.12890608442500787No Hit
GAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCC525700.12880317908885167No Hit
CACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG516030.1264339062302076No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG513080.1257111187500628No Hit
ATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGG511210.12525294499146253No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG505010.12373386621963281No Hit
CCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTC503870.12345455173578025No Hit
CCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTG503520.12336879728898342No Hit
CCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGA499970.12249900218575835No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA493070.12080841452033494No Hit
CGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAT489750.11999497233929066No Hit
GTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCG484140.1186204510634899No Hit
GTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGG481480.11796871726783394No Hit
CGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC481350.11793686561616652No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA479250.1174223389353855No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC477900.11709157178345483No Hit
CCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGGGTCCGCCG477080.11689066136524509No Hit
CTCGGATAGCCGGTCCCCCGCCTGTCCCCGCCGGCGGGCCGCCCCCCCCC476680.11679265628319158No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG471940.11563129606085723No Hit
GGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCC470040.11516577192110297No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCG467360.11450913787134431No Hit
CGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCA465590.11407546538325745No Hit
GGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGA465260.1139946111905633No Hit
CCCGAATCCGGAGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTA459860.11267154258284064No Hit
CGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAG451140.11053503179407369No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATG450750.11043947683907149No Hit
GGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGT440500.10792809661144978No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGA439000.10756057755374904No Hit
CGACGCACCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGG430410.1054559184166495No Hit
CGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCT429630.1052648085066451No Hit
CGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCGAC425040.10414020019008086No Hit
GGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGAT422470.10351051753788694No Hit
TTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAG416280.10199388889310856No Hit
CGCCCCCCGGCCCCGCGTCCTCCCTCGGGAGGGCGCGCGGGTCGGGGCGG412630.10109959251937009No Hit
CCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGG412190.1009917869291112No Hit
GGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCG409310.10028615033832579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG263650.043.5134663
CTCACCC307700.037.3650442
TTGTCTC302250.033.8934332
AACACGT167100.033.8551253
CTAACAC170350.033.7234951
CTTGTCT312950.033.4053231
TAACACG173750.032.5191422
ACACGTG176750.032.4399954
ACGTGCG178600.032.398916
TGTCTCA318600.032.027633
GTCTCAA325500.031.2751834
TGCGCGA185250.030.711439
CCAGTAG113300.030.2459355
GCCAGTA115450.029.6826384
CCTCACC410300.028.8285791
TGCCAGT122300.028.0592543
CAGTAGC124150.027.8340286
CACGTGC209100.027.5814425
TCAAAGA371600.027.1820957
CAAAGAT541600.026.6410541
Produced by FastQC (version 0.11.4)