FastQCFastQC Report
Tue 25 Mar 2025
SH_dif_3_S1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH_dif_3_S1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40117363
Sequences flagged as poor quality0
Sequence length101
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT2970460.7404424862122667No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA2692830.6712380372558385No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC2503460.6240340373319154No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT2354570.586920431435137No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA1985030.49480570295709614No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT1939900.4835562098136909No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG1457910.36341122421231925No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA1361420.33935929437834683No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1202330.29970314848461No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT1169520.2915246448277271No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA1156240.2882143574591381No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA1130180.28171841703553646No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG1074670.2678815155422853No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC1062310.26480055531067687No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG1038010.2587433276708641No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT1003460.2501310966027353No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG1000380.24936334923110473No Hit
CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA1000290.24934091505466102No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC965250.24060654235922735No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA912660.2274975052572623No Hit
CTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGT851740.21231206049111456No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA831820.207346629438231No Hit
GGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTC792940.19765506521453066No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC782560.19506765686468475No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA781830.1948856907668632No Hit
CTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGC781540.1948134028649889No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGA768830.191645198613877No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC743920.18543591711150106No Hit
CGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCT719480.17934379186388696No Hit
GCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAG714900.17820214155152722No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG696010.17349345718461107No Hit
CCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGA695880.1734610522630812No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATG685820.17095340987392416No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA681870.16996879879667065No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA672610.16766057130923584No Hit
GGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGA666870.16622976938937886No Hit
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCT665040.16577360780168926No Hit
CGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCT662340.16510058250837673No Hit
CTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAA658910.16424559111724268No Hit
GCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC656800.16371963431395029No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA652110.16255056445260374No Hit
AACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG647770.16146873861076064No Hit
GGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGAT644780.1607234254155738No Hit
CTCCGTTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAAT632930.15776959218381328No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC627120.15632134146005558No Hit
CTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTC622250.1551074032458215No Hit
CAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATGGGCGAGCGCCGTT618930.15427983140367427No Hit
CCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCT617550.15393584069820343No Hit
CTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCC611050.15231559462171032No Hit
CCCCGCTCCCCGCCCCCGGAGCCCCGCGGACGCTACGCCGCGACGAGTAG599930.1495437274877713No Hit
CGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATG595410.1484170332930407No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT590600.1472180511964358No Hit
CGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGATATAGACAGCAGGA563920.14056756422399946No Hit
TGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCC561230.13989703161695846No Hit
GTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGTG552930.13782810201159035No Hit
AATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGC547780.1365443685817535No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG540300.13467983925065063No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT532650.13277293425293182No Hit
CACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAG531760.13255108517476585No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTT530690.13228436774371238No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT526660.13127981517628665No Hit
CTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATG524750.1308037120984248No Hit
GGTCAGTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCG522770.13031016021666228No Hit
CGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTC510400.12722670729878233No Hit
CTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGG508830.12683535555415246No Hit
GAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACT507640.12653872588784063No Hit
CGCCTCCTCCCCTCCTCCCGCCCACGCCCCGCTCCCCGCCCCCGGAGCCC497420.12399120051833916No Hit
TGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGT496790.1238341612832329No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT488020.12164807542310296No Hit
GGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCG485860.12110965518845294No Hit
AAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTC485660.12105980146302239No Hit
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGC485120.12092519640435988No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC484230.1207033473261939No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCC481640.12005774158186817No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG475160.11844248087791813No Hit
CGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCT468320.11673748346819307No Hit
GTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCA467900.1166327906447889No Hit
CCCGAATCCGGAGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTA463190.11545873541089927No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA451520.11254977053202625No Hit
GTCAGTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGA450380.11226560429707207No Hit
CGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAA447530.11155518870968663No Hit
TTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGAT445240.11098436355350674No Hit
GCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTT444860.11088964147518868No Hit
CGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAG444730.11085723655365881No Hit
CGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCT443230.11048333361292964No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCT442670.11034374318172409No Hit
TCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGC439490.10955106894737822No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG437790.1091273122812185No Hit
CGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAGCCAAGGG437680.10909989273223167No Hit
CCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTG436040.1086910921837011No Hit
CTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC435870.10864871651708513No Hit
CGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCC433320.10801308151784553No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC432990.10793082287088511No Hit
CGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCG427060.10645265991186907No Hit
CCGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAA425820.10614356681419963No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA424550.1058269956577156No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCC424320.10576966387347046No Hit
CGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTG423950.10567743448142392No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG423720.10562010269717878No Hit
CACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT422350.10527860467797946No Hit
CGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATGAAGGTGAAG420030.104700301462985No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCG412140.1027335719947495No Hit
CAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCC411830.10265629872033215No Hit
GGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATT406780.10139749215321057No Hit
CGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTG405260.10101860383993833No Hit
ATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCT402060.1002209442330494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCCA175800.077.019211
CCTCACC447000.070.561081
TCACCCG505250.062.2715873
CTCACCC512150.061.561452
CACCCGG539250.058.5229844
CTAACAC490850.050.3193441
TAACACG534850.046.1321872
AACACGT539400.045.7032623
ACACGTG543750.045.5577934
ACGTGCG566150.043.8907556
CACGTGC566300.043.81135
CTGCCAG315350.042.8964232
TGCGCGA588150.042.206789
CGTGCGC596600.041.8354267
GTGCGCG620250.040.1134158
GTCTAAC219700.037.611741
GGCGATT2950.037.1972961
TCTAACA230000.035.9191442
TGCCAGT391300.034.564393
TCAGACG138500.032.5453382
Produced by FastQC (version 0.11.4)