FastQCFastQC Report
Tue 25 Mar 2025
SH_dif_1_S8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH_dif_1_S8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46448656
Sequences flagged as poor quality0
Sequence length101
%GC63

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16927913.6444348357463774No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1318000.2837541736406754No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA1173930.2527371297890729No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATGA1171370.2521859835944446No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA1169010.2516778956962716No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG1141360.24572508621132116No Hit
CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA1131810.24366905255557877No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT1085710.23374411522262348No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA1048080.22564269674455167No Hit
GGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGG988540.21282424188979765No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG986540.21239365892524428No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC969820.20879398534157803No Hit
TTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCG967400.20827297995446845No Hit
GGGTAACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTAT905760.19500241298693335No Hit
TATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCG904160.19465794661529065No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC865220.1862744962954364No Hit
GCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAG854970.18406775860210034No Hit
CGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT824590.17752720337053457No Hit
CGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTG802540.17278002618633356No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT796200.1714150781886994No Hit
CCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGAGGGCGCGCGGGT791580.17042043154058106No Hit
CTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGT770860.16595959202780808No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC742160.1597807264864671No Hit
GGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTC740190.15935660226638204No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG736410.15854280046337615No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC729740.15710680627659065No Hit
GGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGAT715620.1540668905468438No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA686500.14779760258294664No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG685410.14756293486726504No Hit
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGC677370.1458319913497605No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCG666640.1435219137449316No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT665210.14321404692527595No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAG661340.14238086888886517No Hit
GGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCT660400.14217849489552506No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG650270.13999759218006222No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG629650.1355582818155169No Hit
CCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGA628820.13537958988522725No Hit
GGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTC621940.13389838448716362No Hit
CGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAG617720.13298985443195602No Hit
GGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCG617570.1329575607096145No Hit
CTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACT608410.13098549073196003No Hit
TCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGA574250.12363113369738836No Hit
GGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGA566610.12198630677279446No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG564260.12148037178944425No Hit
CAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAG561400.1208646381501329No Hit
CGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAT552780.11900882557290784No Hit
CTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCT551640.11876339328311245No Hit
CTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAG550780.11857824260835448No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC537130.11563951387527768No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG529320.11395808739869676No Hit
CGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAGCCAAGGG524920.11301080487667932No Hit
CAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTC518190.1115618932009572No Hit
CAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCA511020.11001825327303334No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA509100.1096048936270621No Hit
CGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAA503380.10837342634843945No Hit
CGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCGAC499930.10763067073458488No Hit
GGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCG493640.10627648731106451No Hit
CGCACCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCT490770.10565860075693041No Hit
CGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC489310.10534427519280644No Hit
GGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATT485510.10452616756015502No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA485160.10445081554135817No Hit
CGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG484720.10435608728915644No Hit
GGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCT482900.10396425679141286No Hit
AATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGC481770.10372097741644022No Hit
GGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGT481280.10361548459012462No Hit
CGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTAC479140.10315476081805251No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA476700.1026294496012974No Hit
GAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTC475810.10243784018207115No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA474990.10226130116660426No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATG474270.10210629129936503No Hit
CGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAA473390.10191683479496155No Hit
CGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATG471050.10141305272643411No Hit
CTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGT468320.10082530697981874No Hit
GGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGA468040.10076502536478127No Hit
CGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAAT465930.10031076033717747No Hit
GGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTC464770.10006102221773651No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCTC381150.031.988422
CTTGTCT392350.031.5449751
TCACCCG433350.031.485693
TGTCTCA393200.030.9762673
GTCTCAA397800.030.5940764
CTCACCC456400.029.7748262
GGGTAAT17800.028.6812211
CCTCACC485600.028.2876471
TCAAAGA436550.027.7537277
CTCAAAG441650.027.5458036
CGGGAAT13600.025.9613151
TATACGC2058.4946805E-1025.7575657
CTAACAC107900.025.0723761
ACCTCAC280800.024.508686
AACACGT110100.023.9507483
ACACGTG114000.023.6351994
GGCGATT11300.023.2229881
AACCTCA301850.022.8628235
ATATACG2103.189234E-822.7897116
AAAGATT566550.022.6630089
Produced by FastQC (version 0.11.4)